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Mapping QTLs for rice (Oryza sativa L.) grain protein content via chromosome segment substitution lines

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Abstract

Grain protein content (GPC) is an important component of rice nutritional quality and influences the physical–chemical properties and taste of cooked rice. Quantitative trait loci (QTLs) for GPC in rice (Oryza sativa L.) were analyzed using chromosome segment substitution lines (CSSLs) developed from 9311 (used as the recipient) and Nipponbare (used as the donor). Using the substitution map strategy, we detected a total of 22 QTLs across all twelve chromosomes except chromosome 1. In addition, 13 QTLs were detected in both years, and 17 QTLs were consistent with previously reported GPC QTLs. Among them, qGPC4, which located between RM6748 and RM348 within the boundary of 30.45–33.95 Mb, was at the same position or flanked by several previous QTLs. qGPC6-1 was mapped to a QTL cluster where the Waxy gene is located. With the same Waxyb alleles of 9311 and Nipponbare, the effect of Waxy on GPC could be removed, and there should exist a true stable GPC QTL near Waxy. Four novel and stable QTLs for GPC were identified in this research. These stable and novel QTLs could serve as candidate genes for future research, and CSSLs are suitable for mapping GPC QTLs in rice note.

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Abbreviations

GPC:

Grain protein content

QTL:

Quantitative trait loci

CSSLs:

Chromosome segment substitution lines

DNA:

Deoxyribonucleic acid

RH:

Relative humidity

SSR:

Simple sequence repeats

DH:

Double haploids

RILs:

Recombinant inbred lines

SCSSs:

Single chromosome segment substitution lines

Mb:

1000 Kilo base

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Acknowledgements

We thank Dr. Chuangen Lv, JAAS, for kindly suggestions of writing.

Funding

This work was supported by grants from the Jiangsu Science and Technology Development Program (BE2019375); the Natural Science Foundation of Jiangsu Province of China (BK20180302); the Agriculture Research System of China (CARS-01–67); the Jiangsu Agriculture Science and Technology Innovation Fund (CX (20)2002); and the Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, China.

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Authors and Affiliations

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    L. Zhao, C.-F. Zhao, L.-H. Zhou, S. Yao, Q.-Y. Zhao, T. Chen, Z. Zhu, Y.-D. Zhang & C.-L. Wang

  2. College of Agriculture, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China

    L. Zhao & C.-L. Wang

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  1. L. Zhao
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  2. C.-F. Zhao
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  3. L.-H. Zhou
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  4. S. Yao
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  5. Q.-Y. Zhao
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  6. T. Chen
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  7. Z. Zhu
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  9. C.-L. Wang
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Contributions

L. Z., C. Z. and C. W. designed the experiments. L. Z. and S. Y. prepared and the powers of grain and measured the nitrogen contents of the grain. T. C. and Z. Z. managed the planting of materials in field. Q. Z. performed analyses. L. Z. wrote the manuscript which was modified by Y. Z. All authors read the manuscript.

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Correspondence to Y.-D. Zhang or C.-L. Wang.

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All data referenced in this manuscript are available from the corresponding author on reasonable request.

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Communicated by I. Molnár.

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Zhao, L., Zhao, CF., Zhou, LH. et al. Mapping QTLs for rice (Oryza sativa L.) grain protein content via chromosome segment substitution lines. CEREAL RESEARCH COMMUNICATIONS 50, 699–708 (2022). https://doi.org/10.1007/s42976-021-00237-y

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