Abstract
The fruits of P. philadelphica are of economic value in Mexico. The plants grow wild or cultivated in traditional farming systems. They are also produced as monoculture. Between the wild and cultivated pools, morphological and physiological differences exist. Plant breeding and the shift towards monoculture could modify the plastome sequence and influence the expression of certain genes. Our objectives were threefold. First, we obtained the complete sequence of the domesticated pool plastome. Then, we compared this to the plastome from the wild pool. Finally, we identified the changes that could be related to the domestication process. The plastome of the Manzano race was sequenced, characterized and compared with the plastome of the Milpero (wild) race. The plastomes had the typical circular and quadripartite structure but differed by 52 bp (domesticated 156,856 bp, wild 156,804 bp). A total of 115 genes and five pseudogenes were identified. In addition, 29 SNPs were detected in coding regions along with 19 polymorphic microsatellites. The complete sequences were highly conserved with an identity of 99.6%. No major differences were found in the coding regions that could be attributed to selection and the change from wild to monoculture. InDels in non-coding regions produced more variation. The variation observed was similar to that detected in other managed species.
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Acknowledgements
This work was supported by UDG and CONACyT-Laboratorio Nacional de Identificación y Caracterización Vegetal (LaniVeg) [Grant No. 293833], Universidad de Guadalajara [Grant Prosni-2018 to OVP] and CONACyT-México through a Masters scholarship for graduate studies in Maestría en Biosistemática y Manejo de Recursos Naturales y Agrícolas (BIMARENA) [Grant No. 928518 awarded to ISP].
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OVP, PZT and ISP conceived and designed the study. ISP, OVP, JPA and AR led the writing. ISP developed the molecular methods, sequencing libraries and bioinformatics analyses. MPZT supported the laboratory work and JPA advised on bioinformatics analysis. All authors reviewed drafts of the paper and approved the final manuscript.
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Sandoval-Padilla, I., Pérez-Alquicira, J., Rodríguez, A. et al. The plastome of the husk tomato (Physalis philadelphica Lam., Solanaceae): a comparative analysis between wild and cultivated pools. Genet Resour Crop Evol 69, 1391–1405 (2022). https://doi.org/10.1007/s10722-021-01334-x
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DOI: https://doi.org/10.1007/s10722-021-01334-x