Abstract
The present study was aimed to evaluate the ability of Meyerozyma guilliermondii P14 to produce bioethanol from pretreated biomass of giant reed (Arundo donax). The wild yeast was isolated from its natural habitat and identified by phylogenetic analysis. Arundo donax biomass was sequentially pretreated by acid and alkali treatments to break the recalcitrant structure. The pretreated biomass was analysed by FTIR spectroscopy and scanning electron microscopy to observe the structural changes in the biomass. Pretreated biomass was then enzymatically saccharified, and the resultant broth was used to produce ethanol by M. guilliermondii P14. The fermentation parameters such as pH, temperature, composition of media, and agitation rate were optimized prior to saccharified broth-based fermentations. The results of this study revealed that the dual pretreatment of biomass could make 90.56% cellulose accessible for enzymatic attack. Subsequently, saccharification of pretreated biomass with two enzyme variants resulted in conversion efficiency of 85–87%. Fermentation of saccharified broth resulted in 32.89 g/L final ethanol concentration at 72 h. This has corresponded to 0.48 g/g of ethanol yield and 0.456 g/L/h volumetric productivity. M. guilliermondii is known to utilize both pentose and hexose sugars unlike Saccharomyces cerevisiae which makes it a major player in cellulosic ethanol production. This study provides a significant evidence to the information pool of second-generation bioethanol production from unconventional glucose fermenting yeasts.
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References
Bhardwaj N, Kumar B, Verma P (2020) Microwave-assisted pretreatment using alkali metal salt in combination with ortho-phosphoric acid for generation of enhanced sugar and bioethanol. Biomass Conv Bioref. https://doi.org/10.1007/s13399-020-00640-1
Boonchuay P, Techapun C, Leksawasdi N, Seesuriyachan P, Hanmoungjai P, Watanabe M, Srisupa S, Chaiyaso T (2021) Bioethanol Production from cellulose-rich corncob residue by the thermo-tolerant Saccharomyces cerevisiae TC-5. J Fungi 7(7):547
Brito JQA, Dias FS, Cunha S, Ramos LP, Teixeira LSG (2019) Multiple response optimization of alkaline pretreatment of sisal fiber (Agave sisalana) assisted by ultrasound. Biotechnol Prog 35(3):e2802
Clauser NM, González G, Mendieta CM, Kruyeniski J, Area MC, Vallejos ME (2021) Biomass waste as sustainable raw material for energy and fuels. Sustainability 13(2):794
da Silveira FA, de Oliveira Soares DL, Bang KW et al (2020) Assessment of ethanol tolerance of Kluyveromyces marxianus CCT 7735 selected by adaptive laboratory evolution. Appl Microbiol Biotechnol 104:7483–7494
Danelli T, Sepulcri A, Masetti G, Colombo F, Sangiorgio S, Cassani E, Anelli S, Adani F, Pilu R (2021) Arundo donax L. biomass production in a polluted area: effects of two harvest timings on heavy metals uptake. Appl Sci 11(3):1147
Dasgupta D, Suman SK, Pandey D, Ghosh D, Khan K, Agrawal D, Jain RK, Vadde VT, Adhikari DK (2013) Design and optimization of ethanol production from bagasse pith hydrolysate by a thermotolerant yeast Kluyveromyces sp. IIPE453 using response surface methodology. SpringerPlus 2:159
De Bari I, Liuzzi F, Villone A, Braccio G (2013) Hydrolysis of concentrated suspensions of steam pretreated Arundo donax. Appl Energy 102:179–189
Deng L, Moore DF (2009) Composite likelihood modeling of neighboring site correlations of DNA sequence substitution rates. Stat Appl Genet Mol Biol 8: 10.2202/1544–1615.1391.
Eblaghi M, Niakousari M, Sarshar M, Mesbahi GR (2016) Combining ultrasound with mild alkaline solutions as an effective pretreatment to boost the release of sugar trapped in sugarcane bagasse for bioethanol production. J Food Process Eng 39:273–282
Ercan Y, Irfan T, Mustafa K (2013) Optimization of ethanol production from carob pod extract using immobilized Saccharomyces cerevisiae cells in a stirred tank bioreactor. Bioresour Technol 135:365–371
Fabricio MF, Valente P, Záchia, Ayub MA (2019) Oleaginous yeast Meyerozymaguilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids. Biotechnol Prog 35(6):e2895
Fan C, Qi K, Xia X-X, Zhong J-J (2013) Efficient ethanol production from corncob residues by repeated fermentation of an adapted yeast. Bioresour Technol 136:309–315
Farwick A, Bruder S, Schadeweg V, Oreb M, Boles E (2014) Engineering of yeast hexose transporters to transport d-xylose without inhibition by d-glucose. Proc Nat Acad Sci USA 111:5159–5164
Fiore V, Piperopoulos E, Calabrese L (2019) Assessment of Arundo donax fibers for oil spill recovery applications. Fibers 7(9):75
García-Torreiro M, López-Abelairas M, Lu-Chau TA, Lema JM (2016) Fungal pretreatment of agricultural residues for bioethanol production. Ind Crops Prod 89:486–492
Gomes D, Cruz M, de Resende M, Ribeiro E, Teixeira J, Domingues L (2021) Very high gravity bioethanol revisited: main challenges and advances. Fermentation 7(1):38
Kaur A, Kuhad RC (2019) Valorization of rice straw for ethanol production and lignin recovery using combined acid-alkali pre-treatment. Bioenerg Res 12:570–582
Kou L, Song Y, Zhang X, Tan T (2017) Comparison of four types of energy grasses as lignocellulosic feedstock for the production of bio-ethanol. Bioresour Technol 241:424–429
Lemões JL, Lemons e Silva CF, Avila SPF, Montero CRS, Anjos e Silva SD, Samios D, Peralba MCR (2018) Chemical pretreatment of Arundo donax L. for second-generation ethanol production. Electron J Biotechnol 31:67–74
Lemons e Silva CF, Schirmer MA, Maeda RN, Barcelos CA, Pereira N (2015) Potential of giant reed (Arundo donax L.) for second generation ethanol production. Electron J Biotechnol 18(1):10–15
Li H, Shen Y, Wu M, Hou J, Jiao C, Li Z, Liu X, Bao X (2016) Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production. Bioresour Bioprocess 3(1):51
Liu GL, Fu GY, Chi Z, Chi Z-M (2014) Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin. Appl Microbiol Biotechnol 98:9129–9138
Loaces I, Schein S, Noya F (2017) Ethanol production by Escherichia coli from Arundo donax biomass under SSF, SHF or CBP process configurations and in situ production of a multifunctional glucanase and xylanase. Bioresour Technol 224:307–313
Maciel MJM, Barbosa KS, Barbosa LAC, Filho SA, Procopio REL (2017) Ethanol production from sugarcane bagasse hydrolysate using newly adapted strains of Meyerozyma guilliermondi CBA-524 and Pichia kudriavzevii CBA-519. Indian J Microbiol Res 4(2):182–186
Martini C, Tauk-Tornisielo SM, Codato CB, Bastos RG, Ceccato-Antonini SR (2016) A strain of Meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media. World J Microbiol Biotechnol 32:80
Mo W, Wang M, Zhan R, Yu Y, He Y, Lu H (2019) Kluyveromyces marxianus developing ethanol tolerance during adaptive evolution with significant improvements of multiple pathways. Biotechnol Biofuels 12:63
Modenbach AA, Nokes SE (2014) Effects of sodium hydroxide pretreatment on structural components of biomass. Trans ASABE 57(4):1187–1198
Ogbonda KH, Kiin-Kabari DB (2013) Effect of temperature and pH on ethanol production by a Blastomyces species isolated from the intestine of oil palm weevil (Rhynchophorus palmarum, coleoptera). Afr J Biotechnol 12(6):588–591
Pilu R, Manca A, Landoni M (2013) Arundo donax as an energy crop: pros and cons of the utilization of this perennial plant. Maydica 58:54–59
Qi K, Xia X-X, Zhong J-J (2015) Enhanced anti-oxidative activity and lignocellulosic ethanol production by biotin addition to medium in Pichia guilliermondii fermentation. Bioresour Technol 189:36–43
Ramadoss G, Muthukumar K (2016) Mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production. Ultrason Sonochem 28:207–217
Romaní A, Garrote G, Ballesteros I, Ballesteros M (2013) Second generation bioethanol from steam exploded Eucalyptus globulus wood. Fuel 111:66–74
Ruchala J, Kurylenko OO, Dmytruk KV, Sibirny AA (2020) Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha). J Ind Microbiol Biotechnol 47:109–132
Rulli M, Bellomi D, Cazzoli A, Carolis GD, D’Odorico P (2016) The water-land-food nexus of first-generation biofuels. Sci Rep 6:22521
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425
Scordia D, Cosentino SL, Jeffries TW (2013) Effectiveness of dilute oxalic acid pretreatment of Miscanthus giganteus biomass for ethanol production. Biomass Bioenerg 59:540–548
Scordia D, Cosentino SL, Lee J-W, Jeffries TW (2011) Dilute oxalic acid pretreatment for biorefining giant reed (Arundo donax L.). Biomass Bioenerg 35(7):3018–3024
UNCTAD (2016) Second generation biofuel markets: state of play, trade and developing countries perspectives. https://unctad.org/system/files/official-document/ditcted2015d8_en.pdf. Accessed 23 Feb 2016
Selim KA, El-Ghwas DE, Easa SM, Hassan MIA (2018) Bioethanol a microbial biofuel metabolite; new insights of yeasts metabolic engineering. Fermentation 4(1):16
Shatalov AA, Pereira H (2012) Xylose production from giant reed (Arundo donax L.): modeling and optimization of dilute acid hydrolysis. Carbohydr Polym 87(1):210–217
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2012) Determination of structural carbohydrates and lignin in biomass. Lab Anal Proced
Sudhakar MP, Jegatheesan A, Poonam C, Perumal K, Arunkumar K (2017) Biosaccharification and ethanol production from spent seaweed biomass using marine bacteria and yeast. Renew Energ 105:133–139
Swain MR, Krishnan C (2015) Improved conversion of rice straw to ethanol and xylitol by combination of moderate temperature ammonia pretreatment and sequential fermentation using Candida tropicalis. Ind Crops Prod 77:1039–1046
Wang C, Bao X, Li Y, Jiao C, Hou J, Zhang Q, Zhang W, Liu W, Shen Y (2015) Cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization. Metab Eng 30:79–88
Wang Z, Li J, Liu J, Tian X, Zhang D, Wang Q (2021) Management of blue mold (Penicilliumitalicum) on mandarin fruit with a combination of the yeast, Meyerozymaguilliermondii and an alginate oligosaccharide. Biol Control 152:104451
Webster R, Driever S, Kromdijk J et al (2016) High C3 photosynthetic capacity and high intrinsic water use efficiency underlies the high productivity of the bioenergy grass Arundo donax. Sci Rep 6:20694
Yan J, Wei Z, Wang Q, He M, Li S, Irbis C (2015) Bioethanol production from sodium hydroxide/hydrogen peroxide-pretreated water hyacinth via simultaneous saccharification and fermentation with a newly isolated thermotolerant Kluyveromyces marxianus strain. Bioresour Technol 193:103–109
Yan W, Gao H, Qian X, Jiang Y, Zhou J, Dong W, Xin F, Zhang W, Jiang M (2021) Biotechnological applications of the non-conventional yeast Meyerozymaguilliermondii. Biotechnol Adv 46:107674
Zabed H, Faruq G, Sahu JN, Azirun MS, Hashim R, Boyce AN (2014) Bioethanol production from fermentable sugar juice. Sci World J 2014:957102
Zhao Y, Huang L, Chen Y (2017) Biochars derived from giant reed (Arundo donax L.) with different treatment: characterization and ammonium adsorption potential. Environ Sci Pollut Res 24:25889–25898
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The authors acknowledge the financial support and infrastructure provided by the Department of Biotechnology, Ministry of Science and Technology, India, to conduct this project. We also thank the Chief Executive Officer of CIAB for the continuous motivation and support.
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Sidana, A., Kaur, S. & Yadav, S.K. Assessment of the ability of Meyerozyma guilliermondii P14 to produce second-generation bioethanol from giant reed (Arundo donax) biomass. Biomass Conv. Bioref. 13, 16723–16735 (2023). https://doi.org/10.1007/s13399-021-02211-4
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DOI: https://doi.org/10.1007/s13399-021-02211-4