Abstract
A set of 32 maize hybrids were evaluated under excessive soil moisture (ESM) stress. The plants were subjected to waterlogging for 12 days at the flowering stage by maintaining 3–5 cm water level. Physiological and biochemical traits were examined to analyze plants’ response to waterlogging stress. The chlorophyll a, b and total chlorophyll content declined due to ESM stress, and the decrease was relatively higher in the case of susceptible hybrids. The decrease in chlorophyll content had shown a significant impact on total carbohydrate content, but the tolerant hybrids thrive better under stress with a capacity to maintain higher carbohydrate concentration. Proline accumulation was enhanced in all hybrids in response to the above stress, but it was tremendously increased in tolerant hybrids to offer osmotic protection compared to the sensitive genotypes. Total chlorophyll, chlorophyll-a, carbohydrate, proline as well as an increase in proline content in response to stress, revealed a significant positive association with seed yield, while percentage decline in chlorophyll, decrease in carbohydrate and senescence percentage maintained the reverse trend. Further, the chlorophyll ‘a' followed by an increase in proline content can be considered as important parameters for assessing tolerance to ESM stress owing to their high positive direct effects on seed yield.
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References
Al-Jibouri HA, Miller PA, Robinson HF (1958) Genotypic and environmental variances and covariances in an upland Cotton cross of interspecific origin 1. Agron J 50(10):633–636. https://doi.org/10.2134/agronj1958.00021962005000100020x
Araki H, Hamada A, Hossain MA, Takahashi T (2012) Waterlogging at jointing and/or after anthesis in wheat induces early leaf senescence and impairs grain filling. Field Crop Res 137:27–36. https://doi.org/10.1016/j.fcr.2012.09.006
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase Beta Vulgaris Plant Physiol 24(1):1. https://doi.org/10.1104/pp.24.1.1
Baran WS, Singh BB (2002) Effect of waterlogging on growth, chlorophylls and saccharides content in maize genotypes. Indian J Plant Physiol 7:246–251
Barickman TC, Simpson CR, Sams CE (2019) Waterlogging causes early modification in the physiological performance, carotenoids, chlorophylls, proline, and soluble sugars of cucumber plants. Plants 8(6):160. https://doi.org/10.3390/plants8060160
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207. https://doi.org/10.1007/BF00018060
Bin Y, Qiujie D (1995) Ameliorative effects of exogenous active oxygen scavengers on waterlogging injury of corn leaves. Acta Agric Boreall: Sinica (china) 10:51–55
Campbell MT, Proctor CA, Dou Y, Schmitz AJ, Phansak P, Kruger GR, Zhang C, Walia H (2015) Genetic and molecular characterization of submergence response identifies Subtol6 as a major submergence tolerance locus in maize. PLoS ONE 10(3):e0120385. https://doi.org/10.1371/journal.pone.0120385
DACNET (2020) Agricultural statistics at a glance 2019. Directorate of Economics and Statistics. Published by Department of Agriculture, Cooperation and Farmers Welfare, New Delhi. Ministry of Agriculture and Farmers Welfare, Government of India, pp. 62–121
Dar MI, Naikoo MI, Rehman F, Naushin F, Khan FA (2016) Proline accumulation in plants: roles in stress tolerance and plant development. In: Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi. pp. 155–166. https://doi.org/10.1007/978-81-322-2616-1_9
Dewey DR, Lu K (1959) A correlation and path-coefficient analysis of components of crested wheatgrass seed production 1. Agron J 51(9):515–518. https://doi.org/10.2134/agronj1959.00021962005100090002x
Drew MC, Jackson MB, Giffard SC (1979) Ethylene-promoted adventitious rooting and development of cortical air spaces (aerenchyma) in roots may be adaptive responses to flooding in Zea mays L. Planta 147(1):83–88. https://doi.org/10.1007/BF00384595
FAOSTAT (2018) Food and Agricultural Organization of the United Nations (FAO), FAO Statistical Database. http://faostat.fao.org. Accessed July 18 2020
Fausey NR, VanToai TT, McDonald MB (1985) Response of ten corn cultivars to flooding. Trans ASAE 28(6):1794–1797. https://doi.org/10.13031/2013.32520
Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments: a review. Plant Signal Behav 7(11):1456–1466. https://doi.org/10.4161/psb.21949
IIMR (2021) India maize scenario (2021). ICAR–Indian Institute of Maize Research (ICAR-IIMR) website. https://iimr.icar.gov.in/india-maze-scenario/. Accessed April 20 2021
Irfan M, Hayat S, Hayat Q, Afroz S, Ahmad A (2010) Physiological and biochemical changes in plants under waterlogging. Protoplasma 241(1–4):3–17. https://doi.org/10.1007/s00709-009-0098-8
Kavi Kishor PB, Sreenivasulu N (2014) Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue? Plant Cell Environ 37(2):300–311. https://doi.org/10.1111/pce.12157
Kavita Kumar A, Kumar M, Kumari S (2018) Morpho-physiological traits and yield of some promising maize (Zea mays L.) Hybrids under waterlogging stress. Int J Adv Biol Res 8:22–27
Kennedy RA, Rumpho ME, Fox TC (1992) Anaerobic metabolism in plants. Plant Physiol 100(1):1–6. https://doi.org/10.1104/pp.100.1.1
Lakshmi MS, Jagadev PN, Das S, Lenka D, Swain D, Tripathy SK (2018) Genetic variability and association analysis of maize hybrids under excessive soil moisture condition. Int J Curr Microbiol App Sci 7(9):2935–2941
Lizaso JI, Ritchie JT (1997) Maize shoot and root response to root zone saturation during vegetative growth. Agron J 89(1):125–134. https://doi.org/10.2134/agronj1997.00021962008900010019x
Lone AA, Warsi MZ (2009) Response of maize (Zea mays L) to excess soil moisture (ESM) tolerance at different stages of life cycle. Botany Res Int 2(3):211–217
Manzoor T, Jayalalitha K (2015) Effect of waterlogging on biochemical parameters and yield in maize hybrids. Int J Food Agric Veterin Sci 5(2):92–97
Olgun M, Metin Kumlay A, Cemal Adiguzel M, Caglar A (2008) The effect of waterlogging in wheat (T aestivum L.). Acta Agric Scand Sect B Soil Plant Sci 58(3):193–198. https://doi.org/10.1080/09064710701794024
Panse VG, Sukhatme PV (1953) Experiments in cultivator’s fields. J Indian Soc Agric Stat 5:144–160
Prasanna YL, Ramarao G (2014) Effect of waterlogging on physiological and biochemical parameters and seed yield in greengram genotypes. Int J Food Agric Veterinary Sci 4(2):176–183
Rai RK, Srivastava JP, Shahi JP (2004) Effect of waterlogging on some biochemical parameters during early growth stages of maize. Indian J Plant Physiol 9:65–68
Ren B, Zhang J, Dong S, Liu P, Zhao B (2016) Effects of waterlogging on leaf mesophyll cell ultrastructure and photosynthetic characteristics of summer maize. PLoS ONE 11(9):e0161424. https://doi.org/10.1371/journal.pone.0161424
Shah NA, Srivastava JP, da Silva JA, Shahi JP (2012) Morphological and yield responses of maize (Zea mays L.) genotypes subjected to root zone excess soil moisture stress. Plant Stress 6(1):59–72
Shin S, Kim SG, Jung GH, Kim CG, Son BY, Kim JT, Kim SG, Yang W, Kwon Y, Shim KB, Woo MO (2016) Evaluation of waterlogging tolerance with the degree of foliar senescence at early vegetative stage of maize (Zea mays L). J Crop Sci Biotechnol 19(5):393–399. https://doi.org/10.1007/s12892-016-0097-1
Singh M, Singh TA (1981) Free proline accumulation in maize (Zea mays L.) subjected to prolonged waterlogging. Plant Soil 59(2):349–351. https://doi.org/10.1007/BF02184206
Smethurst CF, Garnett T, Shabala S (2005) Nutritional and chlorophyll fluorescence responses of lucerne (Medicago sativa) to waterlogging and subsequent recovery. Plant Soil 270(1):31–45. https://doi.org/10.1007/s11104-004-1082-x
Tian L, Bi W, Liu X, Sun L, Li J (2019) Effects of waterlogging stress on the physiological response and grain-filling characteristics of spring maize (Zea mays L.) under field conditions. Acta Physiol Plant. https://doi.org/10.1007/s11738-019-2859-0
Tiryakioğlu M, Karanlik S, Arslan M (2015) Response of bread-wheat seedlings to waterlogging stress. Turk J Agric for 39(5):807–816
Torbert HA, Hoeft RG, Vanden Heuvel RM, Mulvaney RL, Hollinger SE (1993) Short-term excess water impact on corn yield and nitrogen recovery. J Prod Agric 6(3):337–344. https://doi.org/10.2134/jpa1993.0337
Yadav DK, Srivastava JP (2017) Temporal changes in biochemical and antioxidant enzymes activities in maize (Zea mays L.) under waterlogging stress during early growth stage. Int J Curr Microbiol Appl Sci 6:351–362
Yordanova RY, Popova LP (2007) Flooding-induced changes in photosynthesis and oxidative status in maize plants. Acta Physiol Plant 29(6):535–541. https://doi.org/10.1007/s11738-007-0064-z
Zaidi PH, Singh NN (2001) Effect of water logging on growth, biochemical compositions and reproduction in maize (Zea mays L.). J Plant Biol 28(1):61–70
Zaidi PH, Rafique S, Singh NN (2003) Response of maize (Zea mays L.) genotypes to excess soil moisture stress: morpho-physiological effects and basis of tolerance. Eur J Agron 19(3):383–399. https://doi.org/10.1016/S1161-0301(02)00090-4
Zaidi PH, Rafique S, Rai PK, Singh NN, Srinivasan G (2004) Tolerance to excess moisture in maize (Zea mays L.): susceptible crop stages and identification of tolerant genotypes. Field Crops Res 90(2–3):189–202. https://doi.org/10.1016/j.fcr.2004.03.002
Zaidi PH, Rafique S, Singh NN, Srinivasan G (2002) Excess moisture tolerance in maize-progress and challenges. In: Proceedings of 8th Asian regional maize workshop, pp 5–9
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The authors are highly thankful to CIMMYT, Regional Office, Hyderabad for providing seed materials for this investigation.
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Odisha university of agriculture and technology.
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Dash, S.S.S., Lenka, D., Sahoo, J.P. et al. Biochemical characterization of maize (Zea mays L.) hybrids under excessive soil moisture stress. CEREAL RESEARCH COMMUNICATIONS 50, 875–884 (2022). https://doi.org/10.1007/s42976-021-00241-2
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DOI: https://doi.org/10.1007/s42976-021-00241-2