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FLUORESCENT PROTEINS

A blueprint for glow tag engineering

Nature Reviews Chemistry volume 6, pages 7–8 (2022)Cite this article

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In two seminal papers published 25 years ago, Phillips and Remington reported the structure of the green fluorescent protein (GFP). These studies provided a blueprint for the rational engineering of GFP, catalysing efforts that produced a large and growing collection of fluorescent proteins and indicators of cellular activity.

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Fig. 1: The discovery, structure, and development of the green fluorescent protein (GFP).

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Acknowledgements

We thank T. Lambert (Harvard Medical School) for sharing the yearly cumulative numbers of FPs discovered or engineered and N. Shaner (University of California, San Diego) for feedback on this News & Views.

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Authors and Affiliations

  1. Systems, Synthetic, and Physical Biology Program, Rice University, Houston, TX, USA

    Jihwan Lee & François St-Pierre

  2. Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA

    François St-Pierre

  3. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA

    François St-Pierre

  4. Department of Electrical and Computer Engineering, Rice University, Houston, TX, USA

    François St-Pierre

Authors
  1. Jihwan Lee
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  2. François St-Pierre
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Correspondence to François St-Pierre.

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The authors declare no competing interests.

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Lee, J., St-Pierre, F. A blueprint for glow tag engineering. Nat Rev Chem 6, 7–8 (2022). https://doi.org/10.1038/s41570-021-00351-9

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