Genome-wide identification and co-expression analysis of GDSL genes related to suberin formation during fruit russeting in pear

Abstract

Glycine-aspartic acid‑serine-leucine (GDSL) type lipases/esterases genes play critical roles in plant development and are related to the responses to abiotic and biotic stress. However, little is known about the GDSL family in pear (Pyrus spp.). Studies have shown GDSL-domain proteins play key roles in suberin deposition. Suberin deposition in the fruit epidermis, also called russeting, is an important defect that negatively affects consumer's appeal in some fruit species, such as pear, apple and grapevine. Fruit russeting is mainly associated with cuticle microcracking and suberin accumulation in the inner part of the epidermal cell walls. To gain insight into the role of the GDSL gene family in suberin deposition and russet development in pear, we performed a genome-wide characterization of the GDSL family, including their identification, chromosomal localization, phylogenetic relationships, and expression patterns, in different tissues/organs in pear. One hundred and thirteen GDSL-type lipases/esterases genes were identified in the pear genome, and a phylogenetic analysis revealed that GDSL family can be classified into four distinct groups. Thirty GDSL genes were co-expressed with five homolog pear genes of three well-known suberin biosynthesis Arabidopsis genes (AtGPAT5, AtASFT, and AtCYP86B1) in the transcriptional co-expression network during pear fruit development. Among the 30 co-expressed GDSL genes, twelve genes were further analyzed by quantitative Real-time PCR, and the results showed the expression levels of the 12 genes were different between the russet exocarp and green exocarp of sand pear at different fruit development stages. Our study provides a detailed overview of the GDSL gene family and lays the foundation for future functional characterization of GDSL genes in P. bretschneideri.