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The Anthurium APRR2-like Gene Promotes Photosynthetic Pigment Accumulation in Response to Salt Stress

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Abstract

Anthurium andraeanum Lind. is an important ornamental flower in which accumulation of photosynthetic pigments affects both growth status and ornamental quality. ARABIDOPSIS PSEUDO RESPONSE REGULATOR2 (APRR2) transcription factor, encoded by APRR2-like gene, is reported to influence the accumulation of photosynthetic pigments in tomato and cucumber. Although several studies have shown a link between photosynthetic pigment accumulation and stress tolerance, the possible role of APRR2-like genes in abiotic stress responses has been unclear. In this study, an APRR2 ortholog, AaAPRR2, was isolated from leaves of ‘dark green’ mutant in A. andraeanum ‘Sonate’. qRT-PCR showed that AaAPRR2 had higher transcript levels in spathe, root and strong leaf than in other tissues. The analysis of conserved structural domains in the 4 predicted APRR2-like protein sequence showed that AaAPRR2 contains conserved REC superfamily and MYB-like DNA domain. Heterologous overexpression of AaAPRR2 in Arabidopsis increased photosynthetic pigment accumulation and the transcript levels of endogenous genes related to photosynthetic pigment synthesis. Salinity stress analysis of 35S::AaAPRR2 and WT lines suggested that AaAPRR2 improved salt stress tolerance in Arabidopsis. These results demonstrate a link between photosynthetic pigment contents and abiotic stress and may be useful for enhancing plant color and improving ornamental value.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 30871725) and Post-doctoral Foundation of Jiangsu Province (Grant No. 1601156C).

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Authors and Affiliations

  1. Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Department of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Li Jiang, Yanxia Fu, Xingkai Tian, Yuehua Ma, Fadi Chen & Guangdong Wang

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  1. Li Jiang
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  2. Yanxia Fu
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  3. Xingkai Tian
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  4. Yuehua Ma
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  5. Fadi Chen
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  6. Guangdong Wang
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Contributions

Guangdong Wang and Fadi Chen conceived and designed the research. Li Jiang, Yanxia Fu, Xingkai Tian and Yuehua Ma conducted the experiments. Li Jiang, Guangdong Wang and Fadi Chen wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Guangdong Wang.

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Communicated by: Zhi-Liang Zheng

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Jiang, L., Fu, Y., Tian, X. et al. The Anthurium APRR2-like Gene Promotes Photosynthetic Pigment Accumulation in Response to Salt Stress. Tropical Plant Biol. 15, 12–21 (2022). https://doi.org/10.1007/s12042-021-09305-3

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  • DOI: https://doi.org/10.1007/s12042-021-09305-3

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