Abstract
Crucian carp (Carassius auratus) is an important freshwater aquaculture species in China. The study of the genetic diversity of native crucian carp will provide basic data for the protection and utilisation of wild crucian carp resources, as well as allowing us to assess the effect of human activities on the distribution and genetic structure of this species. The genetic diversity analysis of crucian carp in Hongze Lake (Jiangsu Province, eastern China) and its surrounding area was conducted using cyt b and D-loop-containing region partial sequences. From 19 sampling populations, sequencing of cyt b (937 bp) from 725 individuals yielded 23 haplotypes while sequencing of the D-loop-containing region (906 bp) from 714 individuals yielded 43 haplotypes. The averages of haplotype diversity, nucleotide diversity and mean nucleotide difference were 0.511, 0.00801 and 2.851 in cyt b and 0.929, 0.015 and 4.153 in D-loop-containing region. The analysis of molecular variance indicated that the variation within populations was the main source of the total variation. Values of FST based on population pairwise difference showed that the genetic distance of G, H, M and N populations was significantly different from other populations (P < 0.05), and the results of Tajima’s D and Fu’s Fs tests indicated that populations J, K and T experienced a demographic expansion. Phylogenetic analysis indicated that the haplotypes of cyt b, the D-loop-containing region and their concatenated sequences were geographically specific. These results revealed that there was high genetic diversity in C. auratus around Hongze Lake, and human activities have had an effect on animal distribution and genetic structure.
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This work was funded by Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection (No. HSXT2-216) and Top-notch Personnel of Science and Technology in Guizhou Province (2017–096).
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Pan, Z., Zhao, H., Zhu, C. et al. Genetic diversity analysis of crucian carp (Carassius auratus) based on cyt b and D-loop-containing region around Hongze Lake. Environ Biol Fish 104, 1401–1420 (2021). https://doi.org/10.1007/s10641-021-01175-8
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DOI: https://doi.org/10.1007/s10641-021-01175-8