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Identification and molecular characterization of a novel carlavirus infecting rose plants (Rosa chinensis Jacq.)

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Abstract

In the present study, the genome sequence of a potential novel virus, tentatively named "rose virus C" (RVC), was mined from publically available transcriptomic data from a Rosa chinensis plant. The complete genome sequence of RVC consists of 8,386 nt, excluding a 3′ poly(A) tail, and contains five ORFs. Phylogenetic analysis showed that RVC clustered with members of the genus Carlavirus, family Betaflexiviridae. The replicase gene had 48.8-52.1% nt sequence identity to those of other carlaviruses, while the CP gene had 40.4-45.9% nt sequence identity, which is far below the species demarcation cutoff of 72%. The incidence of RVC in rose plants was low (5.4%). Overall, our data suggest that RVC is a novel atypical virus of the genus Carlavirus.

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Acknowledgements

This work was supported by the National Key R & D Program of China (2019YFD1001800) and key inter-governmental projects of the National Key Research and Development Program (2017YFE0110900).

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Fei Xing, Zhixiang Zhang & Shifang Li

  2. Department of Fruit Science, College of Horticulture, China Agricultural University, Beijing, 100193, China

    Dehang Gao & Hongqing Wang

  3. The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA, 5064, Australia

    Nuredin Habili

  4. National Citrus Engineering Research Center, Southwest University, Chongqing, 400712, China

    Mengji Cao

  5. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Shifang Li

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  1. Fei Xing
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  2. Dehang Gao
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  3. Nuredin Habili
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  4. Hongqing Wang
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  5. Zhixiang Zhang
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  6. Mengji Cao
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Contributions

X.F. and L.S.F. designed the experiments and wrote the manuscript. X.F., G.D.H. performed the experiments. H.N. and W.H.Q. made comments and improved the manuscript. C.M.J. conducted the systematic screening of public transcriptomic data. Funding was acquired by Z. Z.X. All authors read and approved the final manuscript.

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Correspondence to Mengji Cao or Shifang Li.

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This article does not contain any studies with animals or human participants performed by any of the authors.

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Xing, F., Gao, D., Habili, N. et al. Identification and molecular characterization of a novel carlavirus infecting rose plants (Rosa chinensis Jacq.). Arch Virol 166, 3499–3502 (2021). https://doi.org/10.1007/s00705-021-05260-2

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  • DOI: https://doi.org/10.1007/s00705-021-05260-2

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