Abstract
ROS act as an signaling molecule in the biological growth and development process. The homeostasis of ROS must be kept by different antioxidant defense mechanisms. Currently, superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) as major antioxidant enzymes are not well understood in cassava (Manihot esculenta). In this research, 7 SODs, 6 CATs, and 6 APXs were identified from the cassava genome by hidden Markov models, which was supported by gene structure, protein motifs, and phylogenetic relationship analyses. SOD, CAT, and APX genes expressed differentially in different tissues of cassava, of which most SODs showed high expression levels. The comprehensive expression profiles revealed the participation of SOD, CAT, APX genes during postharvest physiological deterioration (PPD) of storage root and in response to osmotic stress and ABA as well as Xanthomonas axonopodis infection. Together, this study increases our understanding of cassava SOD, CAT, APX genes feature and their potential function during PPD process and in response to biotic and abiotic stresses in cassava, laying a solid foundation for further gene function analysis in cassava.
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Abbreviations
- ABA:
-
Abscisic acid
- APX:
-
Ascorbate peroxidase
- CAT:
-
Catalase
- FEC:
-
Friable embryogenic calli
- FR:
-
Fibrous root
- H2O2 :
-
Hydrogen peroxide
- HP:
-
Hour postharvest
- MT:
-
Melationin
- OES:
-
Organized embryogenic structure
- PPD:
-
Postharvest physiological deterioration
- RAM:
-
Root apical meristem
- ROS:
-
Reactive oxygen species
- SAM:
-
Shoot apical meristem
- SOD:
-
Superoxide dismutase
- SR:
-
Storage root
- Xam:
-
Xanthomonas axonopodis Pv. Manihotis
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Funding
This research was supported was funded by the Natural Science Foundation of Hainan Province (2019RC290), the National Natural Science Foundation of China (31771859, 31901537), the 2020 Research Program of Sanya Yazhou Bay Science and Technology City (SKJC-2020–02-002), the Guangdong Basic and Applied Basic Research Foundation (2021A1515011236), and the Central Public-Interest Scientific Institution Basal Research Fund for Innovative Research Team Program of CATAS (17CXTD-28, 1630052017017), and the earmarked fund for Modern Agro-industry Technology Research System (nycytx-11).
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Conceptualization, WH.; Methodology and software, SS and YQT; Validation, JZ; Formal analysis, JD, CLW, YY, WWT, MYL, and JHYg; Writing original draft preparation, JZ, SS and YQT; Supervision, JZ, MJC, and WH. All authors have read and agreed to the published version of the manuscript.
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Communicated by: Ray Ming.
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Shang, S., Tang, Y., Dai, J. et al. Genomic Analysis of the Principal Members of Antioxidant Enzymes in Simulated Stresses Response and Postharvest Physiological Deterioration in Cassava. Tropical Plant Biol. 14, 419–428 (2021). https://doi.org/10.1007/s12042-021-09304-4
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DOI: https://doi.org/10.1007/s12042-021-09304-4