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A novel polymycovirus with defective RNA isolated from the entomopathogenic fungus Beauveria bassiana Vuillemin

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Abstract

A novel double-stranded RNA virus was isolated and identified from Beauveria bassiana Vuillemin, derived from the muscardine cadaver of an Ostrinia furnacalis larva in China. The virus contains six dsRNAs, and each viral dsRNA contains only one open reading frame (ORF). As in other polymycoviruses, dsRNA1 encodes an RNA-dependent RNA polymerase (RdRp), dsRNA3 encodes a methyltransferase (MTR), and dsRNA4 encodes a proline-alanine-serine-rich protein. A BLASTp search revealed that the viral RdRp domain showed 79.43%, 79.04%, and 59.05% sequence identity to Beauveria bassiana polymycovirus 2 and 3 (BbPmV-2, BbPmV-3) and Magnaporthe oryzae polymycovirus 1 (MoPmV-1), respectively. Phylogenetic analysis based on RdRp sequences showed that the phylogenetically closest relatives of this virus are BbPmV-2, BbPmV-3, and MoPmV-1. This virus, along with previously ill-defined polymycoviruses (BbPmV-2 and BbPmV-3), appears to belong to an as-yet-unestablished species. The findings further suggest that the virus is a new member of the genus Polymycovirus within the family Polymycoviridae, and we have named it "Beauveria bassiana polymycovirus 4" (BbPmV-4). However, the sixth dsRNA is a defective RNA with the same sequence as that of dsRNA4 except for a deletion of 312 bp from nt 185 to nt 496, but it still contains a complete ORF. To our knowledge, this is the first report of the existence of a defective RNA in a polymycovirus.

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Acknowledgements

This work was supported by the Jilin Agricultural Science and Technology Innovation Project (CXGC202004GH) and the National Key Research and Development Program of China (2017YFD200608).

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Authors and Affiliations

  1. Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, China

    Qin Kang & Qiyun Li

  2. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, 130033, China

    Qin Kang, Le Li, Qiyun Li & Zhengkun Zhang

  3. College of Plant Protection, Jilin Agricultural University, Changchun, 130118, China

    Le Li

  4. Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, 434025, Hubei, China

    Songbai Zhang

  5. State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Jincang Li & Jiatao Xie

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  1. Qin Kang
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  2. Le Li
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  3. Jincang Li
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  5. Jiatao Xie
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  6. Qiyun Li
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  7. Zhengkun Zhang
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Correspondence to Qiyun Li or Zhengkun Zhang.

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Handling Editor: Ioly Kotta-Loizou.

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Kang, Q., Li, L., Li, J. et al. A novel polymycovirus with defective RNA isolated from the entomopathogenic fungus Beauveria bassiana Vuillemin. Arch Virol 166, 3487–3492 (2021). https://doi.org/10.1007/s00705-021-05238-0

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