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Bovine Milk Exosomes Alleviate Cardiac Fibrosis via Enhancing Angiogenesis In Vivo and In Vitro

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Journal of Cardiovascular Translational Research Aims and scope Submit manuscript

Abstract

Cardiac fibrosis is a difficult clinical puzzle without effective therapy. Exosomes play an important role in alleviating cardiac fibrosis via angiogenesis. This research aimed to assess the effect of bovine milk on cardiac fibrosis. The proangiogenic effect of bovine milk exosomes was analyzed both in isoproterenol (ISO)-induced cardiac fibrosis rats in vivo and in human umbilical vein endothelial cells (HUVECs) after oxygen and glucose deprivation (OGD) in vitro. Results indicated that bovine milk exosomes alleviated the extracellular matrix (ECM) deposition and enhanced the cardiac function in cardiac fibrosis rat. The proangiogenic growth factors were significantly enhanced in rats accepted bovine milk exosomes. Meanwhile, bovine milk exosomes ameliorated the motility, migration, and tube-forming ability of HUVECs after OGD in vitro. Bovine milk exosomes alleviate cardiac fibrosis and enhance cardiac function in cardiac fibrosis rats via enhancing angiogenesis. Bovine milk exosomes may represent a potential strategy for the treatment of cardiac fibrosis.

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Abbreviations

ECM:

Extracellular matrix

ISO:

Isoproterenol

PBS:

Phosphate-buffered saline

H&E:

Hematoxylin and eosin

LVIDd:

Left ventricular internal dimension at end-diastole

LVIDs:

Left ventricular internal dimension at end-systole

LVPWs:

Left ventricular posterior wall thickness at end-systole

LVPWd:

Left ventricular posterior wall thickness at end-diastole

SV:

Stroke volume

ESV:

End-systole volume

EDV:

End-diastole volume

CO:

Cardiac output

EF:

Ejection fraction

FS:

Fractional shortening

PCNA:

Proliferating cell nuclear antigen

VEGF:

Vascular endothelial growth factor

HUVEC:

Human umbilical vein endothelial cell

RPMI:

Roswell Park Memorial Institute

FBS:

Fetal bovine serum

OGD:

Oxygen and glucose deprivation

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Acknowledgements

We thank the Department of Medical Ultrastructure, School of Basic Medicine, and the Lab of Biomedical Electronic Microscopy of Higher Research Center, Central South University for assistance with TEM work.

Funding

This research was funded by the Natural Science Foundation of Hunan Province, grant number 2019JJ50936, 2019JJ50950, and the Youth Science Foundation of Xiangya Hospital, grant number 2019Q14.

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Authors and Affiliations

  1. Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, China

    Chengliang Zhang & Jinfang Xiao

  2. Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, Changsha, China

    Chengliang Zhang

  3. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China

    Xiaoxu Lu, Jiajia Hu & Jianqin Yan

  4. Department of Obstetrics, Xiangya Hospital, Central South University, Changsha, China

    Ping Li

  5. Department of Anesthesiology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China

    Xiaomei Ling

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Correspondence to Jinfang Xiao.

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Zhang, C., Lu, X., Hu, J. et al. Bovine Milk Exosomes Alleviate Cardiac Fibrosis via Enhancing Angiogenesis In Vivo and In Vitro. J. of Cardiovasc. Trans. Res. 15, 560–570 (2022). https://doi.org/10.1007/s12265-021-10174-0

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