Anti-fungal activity and preliminary active components separation from ethanol extracts in Saffron (Crocus sativus L.) lateral buds
Introduction
Microbial activities are the primary mode by which many foods spoil and are often responsible for the loss of quality and safety (Mead et al., 1999; McCabe-Sellers and Samuel, 2004). Food deterioration results in food loss and decreases its edible value, thereby incurring significant economic losses in the commercialization phase and poses the risk of food poisoning (Creppy, 2002; Lv et al., 2020; Sengun et al., 2008). According to estimates in China, 20–30 % of food is lost to spoilage each year (Mahmoud, 2019; Ritota and Manzi, 2020). In order to prolong the shelf life of food and keep food fresh, the addition of preservatives is necessary, of which chemical preservatives are extensively used (Abdulmumeen et al., 2012; Messager et al., 2004). However, researchers have found that some chemical preservatives pose serious consequences, including cancer, deformity, and bromatoxism. Thus, finding efficient, safe, steady, and broad-spectrum food preservatives has become imperative to avoid the side effects of chemical preservatives (Hsueh et al., 2005; Lai et al., 2002; Pitten et al., 2003). Natural food preservatives, in accordance with the aforementioned issues (Ben et al., 2007; Carocho et al., 2015), possess promising natural antimicrobial agents with potential applications in the food industry that could control pathogenic microbes. Many plant extracts have recently increased in popularity and are of scientific interest due to their antibacterial and anti-fungal activities (Granato et al., 2017; Fierascu et al., 2018; Tajkarimi et al., 2010). Previous studies have reported the antimicrobial properties of plant extracts containing phenols (Jurd et al., 1971; King et al., 1972; Rawat et al., 2018), alkaloids (Adewole et al., 1994; Nissanka et al., 2001; Yan et al., 2007), flavonoids (Lu et al., 2002; Pistelli and Giorgi, 2012; Sharma and Bharat, 2016), anthraquinone (Kosalec et al., 2013; Locatelli et al., 2011; Wu et al., 2006), coumarin (Arshad et al., 2011; Govori et al., 2010), and saponins (Avato et al., 2006; Elekofehint et al., 2019), among others.
Saffron (Crocus sativus L.) is a flowering plant that belongs to the Iridaceae family (Khazdair et al., 2015), which is produced by dring the long orange-red stigmas of the saffron crocus (Aytekin and Acikgoz, 2008). It is one of the most costly traditional medicine plant products and is sold for 200–1600 USD/kg in the world markets, depending on the quality (Gresta et al., 2009; José Bagur et al., 2017; Sampathu et al., 1984). Pharmacological studies and clinical practices have demonstrated that saffron stigmas contain many active compounds that possess promising biological functions, including its effects on blood circulation, as a sedative analgesic, anticonvulsant, antidepressant, anxiolytic, hypolipidemic, anti-atherogenic, anti-hypertensive, antidiabetic, and anti-cancer properties, among others (Christodoulou et al., 2019; Fernández-Albarral et al., 2020; Ghaffari and Roshanravan, 2019; Hosseini et al., 2018; Kianbakht and Hajiaghaee, 2011; Ríos et al., 2015). Furthermore, the saffron stigma is used as a spice and condiment for food, as well as a dye for thousands of years (Basker and Negbi, 1983; Fernández-Albarral et al., 2020). Due to its potential market prospects, many countries are devoted to saffron production. Saffron is mainly cultivated in the European Mediterranean region and Asia, with Iran as the primary producer (85 %) (Ghaffari and Roshanravan, 2019; Milajerdi and Mahmoudi, 2014).
In China, there are two main steps when cultivating saffron in terms of climate. First, plants flower indoors, and their stigmas are harvested, then plants are cultivated in the field so that daughter corms may form. To obtain higher quantity and quality saffron, their lateral buds must be discarded, which is based on the size of the maternal corms, including one bud (maternal corms < 16 g), two buds (16–25 g), and three buds (> 25 g). Although these products are well above the average stigma yield, saffron lateral buds are usually discarded in saffron culturing. Previous studies have shown that the lateral bud contains several bioactive compounds, including alkaloids, phenolic compounds, kaempferols, and anthraquinone (Serrano-Díaz et al., 2012), which have considerable developmental and utilization value. However, very little research has focused on lateral bud utilization. In this study, discarded saffron lateral buds were used as raw materials, and the anti-fungal activities of the ethanol extracts on six common food-borne pathogenic fungi were assessed. The stability of this activity under various heat and pH conditions was also investigated. Furthermore, the active anti-fungal components were preliminarily separated by silica gel column chromatography technology and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results obtained will provide basic data and technical support for the utilization of saffron resources, as well as supply a potentially efficient and affordable source of natural preservatives for use in food.
Section snippets
Plant materials and chemical reagents
Fresh saffron lateral buds were collected from the growing fields of saffron in Shanxi Agriculture University (Taigu, Shanxi, China) in October 2018 (Fig. 1). A representative and randomized amount of fresh lateral bud material was dried in a ventilated oven (Tianjin Taiste Instrument Co. Ltd., Tianjin, China) at 50 °C to a constant weight, finely ground to a dry powder in a grinder series used in Chinese medicine (Tianjin Taiste Instrument Co. Ltd., Tianjin, China), and stored at 4 °C for
Anti-fungal activity assay
The inhibitory effects of the extracts were tested in six concentrations (10, 5, 2.5, 1.25, 0.625, and 0.3125 mg/mL) on A. niger, P. citrinum, R. nigricans, A. oryzae, T. viride, and S. cerevisiae. Only photos of the tested fungi colonies at the 0.3125 mg/mL concentration are provided (Fig. 2). The colony diameters of the fungi in the treated samples were significantly smaller than the negative control, indicating that the extracts exhibited a potential inhibitory effect against the tested
Discussion
Illness caused by the consumption of contaminated foods has a broad economic and public health impact worldwide (Mead et al., 1999). Many pathogenic microorganisms have been reported as the causal agents of food-borne diseases (McCabe-Sellers and Samuel, 2004). A variety of different chemical and synthetic compounds have been used as antimicrobial agents to inhibit pathogens in food. Due to the potential toxicity and carcinogenicity of chemical food preservatives, there is an increasing demand
Conclusions
In order to expand saffron resource utilization, this study clearly demonstrated the in vitro anti-fungal activities of saffron lateral bud ethanol extracts against six representative food-borne fungi, and the results showed that saffron lateral bud extracts elicited a remarkable anti-fungal effect against six tested fungi, especially for A. niger (83.71 %) and T. viride (97.32 %). Moreover, these inhibitory effects were stable in neutral and acidic pH ranges at temperatures<100 °C. Thus,
CRediT authorship contribution statement
Defu Wang: Funding acquisition, Writing - review & editing. Liyan Cui: Validation, Writing - original draft. Hui Ren: Validation, Writing - original draft. Yufen Wang: Visualization, Investigation. Dandan Long: Supervision, Visualization. Yanbing Niu: Conceptualization, Supervision.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgements
This study was supported by China Agriculture Research System of MOF and MARA (Grant No. CARS-21), National Natural Science Foundation of China (Grant No. 31772130) and Modern Agro-industry Technology Research System (No. 2020-05). The authors would like to express their gratitude to EditSprings (https://www.editsprings.com/) for the expert linguistic services provided.
References (69)
- et al.
Synthesis and antimicrobial properties of some new thiazolyl coumarin derivatives
Eur. J. Med. Chem.
(2011) Essential oils: their antibacterial properties and potential applications in foods-a review
Int. J. Food Microbiol.
(2004)- et al.
Natural food additives: Quo vadis?
Trends Food Sci. Technol.
(2015) Update of survey regulation and toxic effects of mycotoxins in Europe
Toxicol. Lett.
(2002)- et al.
Essential oils to control Alternaria alternate in vitro and in vivo
J. Food Control
(2007) - et al.
In vitro and in vivo evaluation of antioxidant properties of wild-growing plants. A short review
Curr. Opin. Food Sci.
(2018) - et al.
Saffron; an updated review on biological properties with special focus on cardiovascular effects
Biomed. Pharmacother.
(2019) - et al.
An integrated strategy between food chemistry, biology, nutrition, pharmacology, and statistics in the development of functional foods: a proposal
Trends Food Sci. Technol.
(2017) - et al.
Analysis of flowering, stigmas yield and qualitative traits of saffron (Crocus sativus L.) as affected by environmental conditions
Sci. Hortic.
(2009) - et al.
Relationships between antimicrobial use and antimicrobial resistance in Gram-negative bacteria causing nosocomial infections from 1991-2003 at a university hospital in Taiwan
Int. J. Antimicrob. Agents
(2005)
Antimicrobial properties of natural phenols and related compounds II: Cinnamylated phenols and their hydrogenation products
J. Pharm. Sci.
Anthraquinone profile, antioxidant and antimicrobial activity of bark extracts of Rhamnus alaternus, R. fallax, R. intermedia and R. pumila
Food Chem.
An anionic antimicrobial peptide from toad Bombina maxima
Biochem. Bioph. Res. Co.
Food safety: emerging trends in foodborne illness surveillance and prevention
J. Am. Diet. Assoc.
Comparison of two in vivo and two ex vivo tests to assess the antibacterial activity of several antiseptic
J. Hosp. Infect.
Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens
Int. J. Food Microbiol.
ChemInform abstract: Antimicrobial alkaloids from Zanthaxylum tetraspermum and caudatum
Phytochemistry
A standardized test to assess the impact of different organic challenges on the antimicrobial activity of antiseptics
J. Hosp. Infect.
Efficacy of Lippia alba (Mill.) NE Brown essential oil and its monoterpene aldehyde constituents against fungi isolated from some edible legume seeds and aflatoxin B1 production
Int. J. Food Microbiol.
Anti-fungal, aflatoxin inhibition and antioxidant activity of Callistemon lanceolatus (Sm.) Sweet essential oil and its major component 1, 8-cineole against fungal isolates from chickpea seeds
Food Control
Antimicrobial herb and spice compounds in food
Food Control
Food: its preservatives, additives and applications
Int. J. Chem. Biochem. Sci.
Antimicrobial properties of alkaloids from Xanthorhiza simplicissima
J. Pharm. Sci.
Evaluating eco-friendly botanicals (Natural Plant Extracts) as alternatives to synthetic fungicides
Ann. Agric. Environ. Med.
Extracts some plants on controlling green mold of orange and on postharvest quality parameters
World Appl. Sci. J.
Antimicrobial activity of saponins from Medicago sp. :Structure-activity relationship
Phytother. Res.
Hormone and microorganism treatments in the cultivation of Saffron (Crocus sativus L.) plants
Molecules
Uses of saffron
Econ. Bot.
Investigation of some medicinal plants from Tunisia for antimicrobial activities
Pharm. Biol.
Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance
Fitoterapia
Antimicrobial activity of spices
J. Rapid Methods Autom. Microbiol.
Research progress of chemical components and pharmacological effects of non-medicinal parts of Crocus sativus
Chin. J. Chin. Mater. Med.
Preparation, chemical characterization and determination of crocetin’s pharmacokinetics after oral and intravenous administration of saffron (Crocus sativus L.) aqueous extract to C57/BL 6J mice
J. Pharm. Pharmacol.
Multiple range and multiple F test
Biometrics
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These two authors contributed equally to this work and should be considered co-first authors.