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Comprehensive Transcriptome Analyses in Sea Louse Reveal Novel Delousing Drug Responses Through MicroRNA regulation

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Abstract

The role of miRNAs in pharmacological responses through gene regulation related to drug metabolism and the detoxification system has recently been determined for terrestrial species. However, studies on marine ectoparasites have scarcely been conducted to investigate the molecular mechanisms of pesticide resistance. Herein, we explored the sea louse Caligus rogercresseyi miRNome responses exposed to delousing drugs and the interplaying with coding/non-coding RNAs. Drug sensitivity in sea lice was tested by in vitro bioassays for the pesticides azamethiphos, deltamethrin, and cypermethrin. Ectoparasites strains with contrasting susceptibility to these compounds were used. Small-RNA sequencing was conducted, identifying 2776 novel annotated miRNAs, where 163 mature miRNAs were differentially expressed in response to the drug testing. Notably, putative binding sites for miRNAs were found in the ADME genes associated with the drugs’ absorption, distribution, metabolism, and excretion. Interactions between the miRNAs and long non-coding RNAs (lncRNAs) were also found, suggesting putative molecular gene regulation mechanisms. This study reports putative miRNAs correlated to the coding/non-coding RNAs modulation, revealing novel pharmacological mechanisms associated with drug resistance in sea lice species.

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Funding

This study was funded by FONDECYT #1210852, FONDECYT #11200813, and FONDAP #15110027, granted by CONICYT-Chile.

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Authors and Affiliations

  1. Interdisciplinary Center for Aquaculture Research, University of Concepción, O’Higgins 1695, Concepción, Chile

    Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Diego Valenzuela-Miranda & Cristian Gallardo-Escárate

  2. Laboratory of Biotechnology and Aquatic Genomics, Department of Oceanography, Center of Biotechnology, Barrio Universitario S/N, Concepción, Chile

    Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Diego Valenzuela-Miranda & Cristian Gallardo-Escárate

Authors
  1. Gustavo Núñez-Acuña
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  2. Valentina Valenzuela-Muñoz
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  3. Diego Valenzuela-Miranda
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  4. Cristian Gallardo-Escárate
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Contributions

G.N., V.V., and C.G. designed the experiment. G.N., V.V., and D.V. conducted the transcriptome analyses. G.N. performed the statistical analyses. G.N. and C.G. led the manuscript writing. All the authors revised and agreed with the final version of the manuscript.

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Correspondence to Cristian Gallardo-Escárate.

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Núñez-Acuña, G., Valenzuela-Muñoz, V., Valenzuela-Miranda, D. et al. Comprehensive Transcriptome Analyses in Sea Louse Reveal Novel Delousing Drug Responses Through MicroRNA regulation. Mar Biotechnol 23, 710–723 (2021). https://doi.org/10.1007/s10126-021-10058-z

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  • DOI: https://doi.org/10.1007/s10126-021-10058-z

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