Research ArticleDirected evolution of lipase A from Bacillus subtilis for the preparation of enantiocomplementary sec-alcohols
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Graphical abstract
Biocatalysts with tailor-made or controllable stereoselectivity are valuable and highly desired in the precision synthesis of chiral compounds. Herein, directed evolution of BSLA provided the best mutant H76 A/N18C with highly reversed (S)-selectivity towards sec-alcohols. A series of enantiocomplementary sec-alcohols can be prepared through the kinetic resolution catalyzed by WT BSLA or mutants, and the highest enantiomeric ratio (E) value is up to >300 for (R)-enantiomer and 178 for (S)-enantiomer, respectively.
Keywords
Lipase A from Bacillus subtilis
Directed evolution
sec-Alcohols
Hydrolysis
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