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Stenotrophomonas nematodicola sp. nov., isolated from Caenorhabditis elegans

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A Correction to this article was published on 07 February 2022

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Abstract

A Gram stain-negative, coccoid rod-shaped, motile by gliding, facultatively aerobic bacterium, designated W5, was isolated from Caenorhabditis elegans samples in Baotianman Natural Reserve (33° 27′ 47′′ N; 111° 48′ 32′′ E), Nanyang, China. The isolate was characterized taxonomically using a polyphasic approach. The 16S rRNA gene of strain W5 exhibited 98.1–99.7% similarity to the 16S rRNA genes of members of the genus Stenotrophomonas, and < 98.0% similarities to those of other bacterial species in the family Lysobacteraceae. The most closely related strains were Stenotrophomonas rhizophila JCM 13333T (99.7%) and Stenotrophomonas bentonitica DSM 103927T (99.2%). The predominant respiratory quinone of the isolate is Q-8. The major fatty acids are iso-C15:0 (38.2%) and antesio-C15:0 (16.6%). The draft genome of strain W5 had a length of 4,402,751 bp and a DNA G + C content of 67.3 mol%. The ANI values between the draft genomes of strain W5 and its closest phylogenetic neighbors S. rhizophila JCM 1333T and S. bentonitica DSM 103927T were 84.7% and 85.0%, respectively. The DDH value between W5 and S. rhizophila JCM 13333T was 30.8%, which was the highest DDH level. We propose that strain W5 represents a novel bacterial species with the name Stenotrophomonas nematodicola sp. nov. and W5 as the type strain. The type strain is W5 (= CPCC 101271T = CGMCC 19401T = KCTC 82903T).

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The DDBJ/EMBL/GenBank accession number for the 16S rRNA gene sequence of strain CPCC 101271T is MT126327; The draft genome sequence of strain CPCC 101271T is WIAY00000000.

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Acknowledgements

The authors thank JCM (Japan Collection of Microorganisms) and DSMZ (German Collection of Microorganisms and Cell Cultures) for providing the reference strains.

Funding

This research was supported by the National Natural Science Foundation of China (NSFC 31670010 and 31570120), the Drug Innovation Major Project (2018ZX09711001-007-001), the National Infrastructure of Microbial Resources (NIMR-2020-3), CAMS Innovation Fund for Medical Sciences (CIFMS) (2016-I2M-2-002) and Innovation Scientists and Technicians Troop Construction Projects (Sustainable Utilization of Energy Microbial Resources) of Henan Province.

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Authors and Affiliations

  1. College of Life Science and Agricultural Engineering, Nanyang Normal University, 1638 Wolong Road, Nanyang, 473061, Henan, China

    Xuyang Wei, Shengwei Lei, Lin Zhang & Qiuhong Niu

  2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China

    Yang Deng & Yuqin Zhang

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  1. Xuyang Wei
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  2. Shengwei Lei
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  3. Yang Deng
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  4. Yuqin Zhang
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  5. Lin Zhang
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  6. Qiuhong Niu
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Contributions

QN designed the experiments. XW and SL carried out the experiments. YD prepared the figures. YZ and LZ designed the project and prepared the manuscript together with QN. All authors reviewed the manuscript.

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Correspondence to Lin Zhang or Qiuhong Niu.

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Communicated by Erko Stackebrandt.

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Wei, X., Lei, S., Deng, Y. et al. Stenotrophomonas nematodicola sp. nov., isolated from Caenorhabditis elegans. Arch Microbiol 203, 6197–6202 (2021). https://doi.org/10.1007/s00203-021-02586-y

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