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Comparison of Enzymatic Activity of Novel Recombinant L-asparaginases of Extremophiles

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Abstract

The activity of novel uncharacterized extremophilic L-asparaginases from the psychrophilic fungi Sclerotinia borealis, the thermoacidophilic crenarchea Acidilobus saccharovorans, and the thermophilic bacteria Melioribacter roseus were studied. Active enzymes were produced via the expression of the native L-asparaginase gene from M. roseus (MrA) and synthetic genes encoding fungal S. borealis (SbA) and archeal A. saccharovorans (AsA) L-asparaginases after codon optimization in Escherichia coli cells. In the study, the maximum specific activity at different temperatures and pH was observed for MrA. The activity of MrA crude extract was highest at 75°С and a pH of 9.0. Metal ions (1 mM) differed in their effects on enzyme activity. Сu2+ and Zn2+ ions completely abolished enzyme activity. Changes in the specific activity of MrA crude extract in the presence of Fe3+, Ni2+, Ca2+, and Mg2+ varied within 5–28%. Our findings show that L-asparaginase of M. roseus may be a promising object for the further study of enzymatic properties and biotechnological applications.

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REFERENCES

  1. Solomon, B., Parihar, N., Ayodele, L., and Hughes, M., J. Blood Disord. Transfus., 2017, vol. 8, p. 24.

    Google Scholar 

  2. Brumano, L.P., Silva, F.V.S., and Costa-Silva, T.A., Front. Bioeng. Biotechnol., 2018, vol. 6, pp. 1–22.

    Article  Google Scholar 

  3. Baskar, G., Subanjalin, J.S., and Aiswarya, R., Int. J. Mod. Sci. Technol., 2016, vol. 6, no. 1, pp. 224–229.

    Google Scholar 

  4. Aiswarya, R. and Baskar, G., Int. J. Food Sci. Technol., 2018, vol. 53, pp. P. 491–498.

  5. Baskar, G. and Aiswarya, R., J. Sci. Food Agricult., 2018, vol. 98, pp. P. 4385–4394.

  6. El-Naggar, N.E.A., El-Ewasy, S.M., and El-Shweihy, N.M., Int. J. Pharmacol., 2014, vol. 10, pp. 182–199.

    Article  CAS  Google Scholar 

  7. Duval, M., Suciu, S., and Ferster, A., Blood, 2002, vol. 99, pp. 2734–2739.

    Article  CAS  PubMed  Google Scholar 

  8. Matsumoto, Y., Nomura, K., and Kanda-Akano, Y., Leuk. Lymphoma, 2003, vol. 44, pp. 879–882.

    Article  CAS  PubMed  Google Scholar 

  9. Sokolov, N.N., El’darov, M.A., Pokrovskaya, M.V., Aleksandrova, S.S., Abakumova, O.Yu., Podobed, O.V., Melik-Nubarov, N.S., Kudryashova, E.V., Grishin, D.V., and Archakov, A.I., Biomed. Khim., 2015, vol. 61, no. 3, pp. 312–324.

    Article  CAS  PubMed  Google Scholar 

  10. Nunes, J.C.F., Cristóvão, R.O., and Freire, M.G., Molecules (Basel, Switzerland), 2020, vol. 25, no. 24, p. 5827.

    Article  CAS  Google Scholar 

  11. Varshosaz, J. and Anvari, N., IET Nanobiotechnol., 2018, vol. 12, no. 4, pp. 466–472.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Krishnapura, P.R., Belur, P.D., and Subramanya, S., Crit. Rev. Microbiol., 2016, vol. 42, no. 5, pp. 470–737.

    Google Scholar 

  13. Sarmiento, F., Peralta, R., and Blamey, J.M., Front. Bioeng. Biotechnol., 2015, vol. 3, p. 148.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Burg, B., Curr. Opin. Microbiol., 2003, vol. 6, no. 3, pp. 213–218.

    Article  PubMed  CAS  Google Scholar 

  15. Hoshino, T., Xiao, N., and Tkachenko, O.B., Mycoscience, 2009, vol. 50, no. 1, pp. 26–38.

    Article  Google Scholar 

  16. Hoshino, T., Terami, F., Tkachenko, O.B., Tojo, M., and Matsumoto, N., Mycoscience, 2010, vol. 51, no. 2, pp. 98–103.

    Article  Google Scholar 

  17. Mardanov, A.V., Svetlitchnyi, V.A., and Beletsky, A.V., Appl. Environ. Microbiol., 2010, vol. 76, no. 16, pp. 5652–5657.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Podosokorskaya, O.A., Kadnikov, V.V., and Gavrilov, S.N., Environ. Microbiol., 2013, vol. 15, no. 6, pp. 1759–1771.

    Article  CAS  PubMed  Google Scholar 

  19. Tiwari, A.K., Rao, J.V., Doriya, K., Kumar, D.S., Qureshi, A., and Ashok, A., Sci. Rep., 2019, vol. 9, p. 1423.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  20. Moguel, I.S., Yamakawa, C.K., Pessoa, A., and Mussatto, S.I., Front. Bioeng. Biotechnol., 2020, vol. 8, p. 576511.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Chohan, S.M. and Rashid, N., J. Biosci. Bioeng., 2013, vol. 116, pp. 438–443.

    Article  CAS  PubMed  Google Scholar 

  22. Altschul, S.F., Madden, T.L., and Schaffer, A.A., Nucleic Acids Res., 1997, vol. 25, no. 17, pp. 3389–3402.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K., Mol. Biol. Evol., 2018, vol. 35, no. 6, pp. 1547–1549.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Lu, G. and Moriyama, E.N., Softw. Rev., 2004, vol. 5, no. 4, pp. 378–388.

    CAS  Google Scholar 

  25. Mardanov, A.V., Beletsky, A.V., Kadnikov, V.V., Ignatov, A.N., and Ravin, N.V., Genome Announc., 2014, vol. 2, no. 1. e01175-13.

    PubMed  PubMed Central  Google Scholar 

  26. Pokrovskaya, M.V., Aleksandrova, S.S., Pokrovsky, V.S., Veselovsky, A.V., Grishin, D.V., Abakumova, O.Y., Podobed, O.V., Mishin, A.A., Zhdanov, D.D., and Sokolov, N.N., Mol. Biotechnol., 2015, vol. 57, no. 3, pp. 192–208.

    Article  CAS  Google Scholar 

  27. Wriston, J.C. and Yellin, T.O., Adv. Enzymol. Rel. Areas Mol. Biol., 2006, vol. 39, pp. 185–248.

    Google Scholar 

  28. Wade, H.E., Robinson, H.K., and Phillips, B.W., J. Gen. Microbiol., 1971, vol. 69, no. 3, pp. 299–312.

    Article  CAS  PubMed  Google Scholar 

  29. Li, A.N., Ding, A.Y., Chen, J., Liu, S.A., Zhang, M., and Li, D.C., Sci. Rep., vol. 8, p. 7915.

  30. Guo, J., Coker, A.R., and Wood, S.P., Acta Crystallogr., 2017, vol. 73, pp. 889–895.

    CAS  Google Scholar 

  31. Safary, A., Moniri, R., Hamzeh-Mivehroud, M., and Dastmalchi, S., BioImpacts, 2019, vol. 9, pp. 15–23.

    Article  CAS  PubMed  Google Scholar 

  32. Kelo, E., Noronkoski, T., Stoineva, I.B., Petkov, D.D., and Mononen, I., FEBS Lett., 2002, vol. 528, pp. 130–132.

    Article  CAS  PubMed  Google Scholar 

  33. Duarte, A.W.F., Barato, M.B., and Nobre, F.S., Polar Biol., 2018, vol. 41, no. 12, pp. 2511–2521.

    Article  Google Scholar 

  34. Farag, A.M., Hassan, S.W., Beltagy, E.A., and El-Shenawy, M.A., Egypt. J. Aquat. Res., 2015, vol. 41, no. 4, pp. 295–302.

    Article  Google Scholar 

  35. Zhgun, A.A., Nuraeva, G.K., Dumina, M.V., Voinova, T.M., Dzhavakhiya, V.V., and Eldarov, M.A., Appl. Biochem. Microbiol., 2019, vol. 55, no. 3, pp. 243–254.

    Article  CAS  Google Scholar 

  36. Gunny, A.A.N., Arbain, D., Jamal, P., and Gumba, R.E., Saudi J. Biol. Sci., 2015, vol. 22, no. 4, pp. 476–483.

    Article  CAS  PubMed  Google Scholar 

  37. Zhgun, A.A., Dumina, M.V., Voinova, T.M., Dzhavakhiya, V.V., and Eldarov, M.A., Appl. Biochem. Microbiol., 2018, vol. 54, no. 2, pp. 188–197.

    Article  CAS  Google Scholar 

  38. Dumina, M.V., Eldarov, M.A., Zdanov, D.D., and Sokolov, N.N., Biomed. Khim., 2020, vol. 66, no. 2, pp. 105–123.

    Article  CAS  PubMed  Google Scholar 

  39. Hatanaka, T., Usuki, H., and Arima, J., Appl. Biochem. Biotechnol., 2011, vol. 163, no. 7, pp. 836–844.

    Article  CAS  PubMed  Google Scholar 

  40. Borisova, A.A., Eldarov, M.A., Zhgun, A.A., Alexandrova, S.S., Omelyanuk, N.M., Sokov, B.N., Berezov, T.T., and Sokolov, N.N., Biomed. Khim., 2003, vol. 49, no. 5, pp. 505–507.

    Google Scholar 

  41. Pritsa, A.A. and Kyriakidis, D.A., Mol. Cell. Biochem., 2001, vol. 216, nos. 1–2, pp. 93–101.

    Article  CAS  PubMed  Google Scholar 

  42. Li, J., Wang, J., and Bachas, L.G., Anal. Chem., 2002, vol. 74, no. 14, pp. 3336–3341.

    Article  CAS  PubMed  Google Scholar 

  43. Curran, M.P., Daniel, R.M., Guy, G.R., and Morgan, H.W., Arch. Biochem. Biophys., 1985, vol. 241, no. 2, pp. 571–576.

    Article  CAS  PubMed  Google Scholar 

  44. Han, S., Jung, J., and Park, W., J. Microbiol. Biotechnol., 2014, vol. 24, no. 8, pp. 1091–1104.

    CAS  Google Scholar 

  45. Warangkar, S.C. and Khobragade, C.N., Enzyme Res., 2010, vol. 2010, pp. 1–10.

    Article  CAS  Google Scholar 

  46. Zuo, S., Zhang, T., Jiang, B., and Mu, W., Extremophiles, 2015, vol. 19, no. 4, pp. 841–851.

    Article  CAS  PubMed  Google Scholar 

  47. Zuo, S., Xue, D., Zhang, T., Jiang, B., and Mu, W., J. Mol. Catal., 2014, vol. 109, no. 14, pp. 122–129.

    Article  CAS  Google Scholar 

  48. Hong, S.J., Lee, Y.H., and Khan, A.R., J. Basic Microbiol., 2014, vol. 163, no. 7, pp. 826–834.

    Google Scholar 

  49. Li, A.N., Ding, A.Y., Chen, J., Liu, S.A., Zhang, M., and Li, D.C., J. Microbiol. Biotechnol., 2007, vol. 17, no. 4, pp. 324–631.

    Google Scholar 

  50. De Azeredo, L.A.I., Freire, D.M.G., Soares, R.M.A., Leite, S.G.F., and Coelho, R.R.R., Enzyme Microb. Technol., 2004, vol. 34, nos. 3–4, pp. 354–358.

    Article  CAS  Google Scholar 

  51. Chohan, S.M., Rashid, N., and Sajed, M., Folia Microbiol., 2019, vol. 64, no. 2, pp. 313–320.

    Article  CAS  Google Scholar 

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Funding

The work was partially supported by the Russian Foundation for Basic Research, project no. 19-08-01112, and by the Program for Basic Scientific Research in the Russian Federation for a long-term period (2021–2030).

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Authors and Affiliations

  1. Research Center of Biotechnology, Russian Academy of Sciences, 117312, Moscow, Russia

    M. V. Dumina, A. A. Zhgun & M. A. El’darov

  2. Institute of Biomedical Chemistry, 119121, Moscow, Russia

    M. V. Pokrovskay, S. S. Aleksandrova, D. D. Zhdanov & N. N. Sokolov

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  1. M. V. Dumina
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  2. A. A. Zhgun
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  6. N. N. Sokolov
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Correspondence to M. V. Dumina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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This article is dedicated to the memory of M. A. El’darov

Translated by E. V. Makeeva

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Dumina, M.V., Zhgun, A.A., Pokrovskay, M.V. et al. Comparison of Enzymatic Activity of Novel Recombinant L-asparaginases of Extremophiles. Appl Biochem Microbiol 57, 594–602 (2021). https://doi.org/10.1134/S0003683821050057

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