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2,4-dichlorophenol Degradation by Indigenous Pseudomonas sp. PKZNSA and Klebsiella pneumoniae KpKZNSA: Kinetics, Enzyme Activity and Catabolic Gene Detection

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Abstract

In this study, two newly isolated 2,4-dichlorophenol(2,4-DCP)-degrading strains, Pseudomonas sp. KZNSA (PKZNSA) and Klebsiella pneumoniae KZNSA (KpKZNSA), were enriched from an activated sludge sample with a known history of contamination with chlorinated organic compounds collected from a wastewater treatment plant located in Durban, South Africa. The strains could use 2,4-DCP as sole carbon and energy source. PKZNSA and KpKZNSA degraded 64 and 49% of 2,4-DCP, with the degradation rate constant of 0.14 and 0.03 mg/L d, respectively. Both PKZNSA and KpKZNSA were found to harbor the catabolic genes that encode the enzymes involved in 2,4-DCP degradation via the ortho-pathway which is further confirmed by the specific enzyme activity assays. The strains did not possess genes that encode the enzyme maleylacetate reductase, which is involved in funneling the last intermediate (maleylacetate) in the pathway into the Krebs cycle. Findings from this study will be helpful in the exploitation of these microorganisms and/or their enzymes in developing bioremediation strategies for the chlorophenol-polluted environment.

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ACKNOWLEDGMENTS

The post-doctoral research fellowship awarded to Dr. A. Kumar by the University of KwaZulu-Natal, Durban, South Africa. The authors are grateful for the MSc scholarship awarded to Boitumelo Setlhare to carry out this study.

Funding

National Research Foundation (NRF), South Africa, Grant nos.: 94036 and 92803.

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Authors and Affiliations

  1. Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, 4000, Durban, Republic of South Africa

    B. Setlhare, A. Kumar, O. A. Aregbesola, M. P. Mokoena & A. O. Olaniran

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  1. B. Setlhare
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  2. A. Kumar
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  3. O. A. Aregbesola
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  4. M. P. Mokoena
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  5. A. O. Olaniran
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Correspondence to A. O. Olaniran.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Setlhare, B., Kumar, A., Aregbesola, O.A. et al. 2,4-dichlorophenol Degradation by Indigenous Pseudomonas sp. PKZNSA and Klebsiella pneumoniae KpKZNSA: Kinetics, Enzyme Activity and Catabolic Gene Detection. Appl Biochem Microbiol 57, 656–665 (2021). https://doi.org/10.1134/S0003683821050148

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