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Streptomyces variabilis Isolate MW091521: a New Microbial Source of Heliomycin

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Abstract

A total of 192 different Streptomyces isolates were recovered from 34 soil samples collected from different localities in Egypt. The cytotoxic activities of culture supernatant of each of the recovered isolate were investigated against Caco-2 cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The Streptomyces isolate coded S19, that showed the highest cytotoxic activity against Caco-2 cell line (CD50 of 26.4 µg/mL), was identified using 16S rRNA gene sequence analysis as Streptomyces variabilis isolate MW091521. The influence of cultural parameters affecting the cytotoxic activity of the respective isolate including the different culture media, initial pH value, temperature and rotation speed was investigated. A maximum cytotoxic activity (CD50 of 13.4 µg/mL) was obtained using the culture medium of soybean meal at an initial pH of 7.0, incubation temperature 28°C and 200 rpm. Thereafter, the fermentation was conducted in a 14 L laboratory fermenter under the previously stated optimum conditions with the aim of extraction, and purification of cytotoxic metabolite(s). Fractionation of the ethyl acetate extract followed by advanced spectroscopic analysis using LC/ESI/MS as well as 1D and 2D NMR experiments yielded the cytotoxic metabolite heliomycin. To the best of our knowledge, this is the first report on the production of heliomycin by Streptomyces variabilis isolate MW091521.

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ACKNOWLEDGMENTS

We hereby acknowledge the Postgraduate and Scientific Research Sector of Ain Shams University for supplying us the chemicals required for performing the practical work and for providing all the required facilities to perform the experiments.

Ethics approval and consent to participate. Not applicable.

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Availability of data and materials. All data generated or analyzed during this study are included in this published article in the main manuscript.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain shams University, Organization of African Unity St. Abbassia, POB: 11566, Cairo, Egypt

    A. S. Abu Zaid, M. A. Yassien & K. M. Aboshanab

  2. Department of Pharmacognosy, Faculty of Pharmacy, Ain shams University, Organization of African Unity St., Abbassia, POB: 11566, Cairo, Egypt

    A. M. Elissawy

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  1. A. S. Abu Zaid
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  2. M. A. Yassien
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  3. K. M. Aboshanab
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Correspondence to K. M. Aboshanab.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Abu Zaid, A.S., Yassien, M.A., Aboshanab, K.M. et al. Streptomyces variabilis Isolate MW091521: a New Microbial Source of Heliomycin. Appl Biochem Microbiol 57, 564–570 (2021). https://doi.org/10.1134/S0003683821050021

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  • DOI: https://doi.org/10.1134/S0003683821050021

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