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RNA interference and crop protection against biotic stresses

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Abstract

RNA interference (RNAi) is a universal phenomenon of RNA silencing or gene silencing with broader implications in important physiological and developmental processes of most eukaryotes, including plants. Small RNA (sRNA) are the critical drivers of the RNAi machinery that ensures down-regulation of the target genes in a homology-dependent manner and includes small-interfering RNAs (siRNAs) and micro RNAs (miRNAs). Plant researchers across the globe have exploited the powerful technique of RNAi to execute targeted suppression of desired genes in important crop plants, with an intent to improve crop protection against pathogens and pests for sustainable crop production. Biotic stresses cause severe losses to the agricultural productivity leading to food insecurity for future generations. RNAi has majorly contributed towards the development of designer crops that are resilient towards the various biotic stresses such as viruses, bacteria, fungi, insect pests, and nematodes. This review summarizes the recent progress made in the RNAi-mediated strategies against these biotic stresses, along with new insights on the future directions in research involving RNAi for crop protection.

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Acknowledgements

We are grateful to the Department of Biotechnology (DBT) and Department of Science and Technology (DST), New Delhi for generous support of the RNAi programmes in the lab of MVR. MVR is grateful to the University Grants Commission (UGC), New Delhi for BSR Faculty Fellowship. RK acknowledges the Dr. D. S. Kothari Postdoctoral Fellowship from UGC. We also thank the UGC for SAP (DRS-III) and DST-FIST (Level 2) programmes and DU-DST PURSE (Phase II) grant.

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  1. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Ranjeet Kaur, Aparajita Choudhury, Sambhavana Chauhan, Arundhati Ghosh, Ruby Tiwari & Manchikatla Venkat Rajam

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  1. Ranjeet Kaur
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Correspondence to Manchikatla Venkat Rajam.

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This review article is dedicated in the honour of Late Prof. S. C. Maheshwari for his invaluable contributions in the field of plant biology and biotechnology.

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Kaur, R., Choudhury, A., Chauhan, S. et al. RNA interference and crop protection against biotic stresses. Physiol Mol Biol Plants 27, 2357–2377 (2021). https://doi.org/10.1007/s12298-021-01064-5

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