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Investigating the Effect of Particle Size on Cellular Uptake by Aggregation-Caused Quenching Probe–Encapsulating Solid Lipid Nanoparticles, Inhaled

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Abstract

Solid lipid nanoparticles (SLN) have been intensively exploited as nanocarriers for pulmonary drug delivery. However, no consensus on the underlying mechanisms between particle sizes and cell uptake behaviors was attained owing to their unclear in vivo fate after inhalation, which hindered the further development of a novel nanoparticle delivery system. Recently, aggregation-caused quenching (ACQ) probes had been developed to monitor the integrity of nanocarriers. In this study, an ACQ probe, P4, was employed to clarify the effect of particle size on SLN cellular uptake. SLN (120, 240, and 480 nm in size), incubated with epithelium cells (A549) and macrophages (RAW 264.7), mainly localized in the cytoplasm in an integrated pattern, showing a positive correlation relationship between particle size and cellular intake. In conclusion, this study established a firm basis to understand the effect of particle size on cellular uptake of nanoparticle systems and promoted the development of novel pulmonary drug delivery systems.

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Acknowledgements

The authors would like to appreciate Ke Xue and Anduo Lu from Sun Yat-Sen University for their help in experimental data collection.

Funding

The study was funded by the National Natural Science Foundation of China (81703431 and 81673375).

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, Guangdong, People’s Republic of China

    Wenhua Wang, Xin Pan & Chuanbin Wu

  2. College of Pharmacy, Jinan University, Guangzhou, 511443, Guangdong, People’s Republic of China

    Beibei Yang, Zhengwei Huang, Ying Huang, Ping Hu & Chuanbin Wu

Authors
  1. Wenhua Wang
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  2. Beibei Yang
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  3. Zhengwei Huang
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  4. Ying Huang
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  5. Ping Hu
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  6. Xin Pan
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  7. Chuanbin Wu
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Correspondence to Zhengwei Huang or Xin Pan.

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Wang, W., Yang, B., Huang, Z. et al. Investigating the Effect of Particle Size on Cellular Uptake by Aggregation-Caused Quenching Probe–Encapsulating Solid Lipid Nanoparticles, Inhaled. J Pharm Innov 17, 1109–1115 (2022). https://doi.org/10.1007/s12247-021-09576-z

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  • DOI: https://doi.org/10.1007/s12247-021-09576-z

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