Elsevier

Photoacoustics

Volume 24, December 2021, 100305
Photoacoustics

Broadband surface plasmon resonance sensor for fast spectroscopic photoacoustic microscopy

https://doi.org/10.1016/j.pacs.2021.100305Get rights and content
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Abstract

High-speed optical-resolution photoacoustic microscopy (OR-PAM), integrating the merits of high spatial resolution and fast imaging acquisition, can observe dynamic processes of the optical absorption-based molecular specificities. However, it remains challenging for the evaluation to morphological and physiological parameters that are closely associated with photoacoustic spectrum due to the inadequate ultrasonic frequency response of the routinely-employed piezoelectric transducer. By utilizing the galvanometer for fast optical scanning and our previously-developed surface plasmon resonance sensor as an unfocused broadband ultrasonic detector, high-speed spectroscopic photoacoustic imaging was accessed in the OR-PAM system, achieving an acoustic bandwidth of ∼125 MHz and B-scan rate at ∼200 Hz over a scanning range of ∼0.5 mm. Our system demonstrated the dynamic imaging of the moving phantoms’ structures and the simultaneous characterization of their photoacoustic spectra over time. Further, fast volumetric imaging and spectroscopic analysis of microanatomic features of a zebrafish eye ex vivo was obtained label-freely.

Keywords

Surface plasmon resonance
Broad bandwidth
Photoacoustic microscopy
Photoacoustic spectroscopic analysis
High-speed imaging

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Fan Yang is a pH.D student at College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China. He received Bachelor degree from the Hebei University of Science and Technology, and received M.S. degree from Shenzhen University. His research focuses on the development of novel photoacoustic imaging technologies.

Guangdi Guo received the M.S. degree from Shenzhen University, Shenzhen, China, in 2020. His research interests include optical sensing and photoacoustic imaging.

Shanguang Zheng received the M.S. degree from Shenzhen University, Shenzhen, China, in 2018. His research interests include optical vortices and applications.

Hui Fang is currently a professor in Nanophotonics Research Center, Institute of Microscale Optoelectronics, Shenzhen University. His research interests include photoacoustic imaging and flow sensing, optical microscopy and spectroscopy, and optical vector beams.

Changjun Min received his B.S. degree and pH.D. degree from University of Science and Technology of China in 2003 and 2008, respectively. During 2008–2011 he was a postdoctoral research fellow in Louisiana State University (USA) and then in Nanyang Technological University (Singapore). During 2011–2014, he worked as Associate Professor in Nankai University (China). Since June 2014, he joined the College of Optoelectronic Engineering in Shenzhen University (China). His research interests include plasmonics, optical sensing/imaging, optical tweezers, vector beams, and metasurface. He has published over 100 journal papers in optics and photonics.

Wei Song received his pH.D. degree from Harbin Institute of Technology, Harbin, China, in 2014. He was an assistant professor with Shenzhen Institute of Advanced Technology from 2015 to 2016. Since 2017, he works as an assistant professor at Nanophotonics Research Centre, Shenzhen University. His main interest is the development and application of novel optical sensing and imaging technologies, including optical surface wave sensing, photoacoustic microscopy, and multimodal imaging.

X.-C. Yuan received BEng and MEng degrees in Optical Engineering from Tianjin University, China, in 1985 and 1988, respectively, and pH.D. degree in Physics from the University of London, King’s College, in 1994. Between 1994 and 1999, he was a research fellow with the Cavendish Laboratory, the University of Cambridge. Between 1999 and 2008, he was a faculty (tenured) in the School of EEE of Nanyang Technological University, Singapore. He joined the Institute of Modern Optics of Nankai University as a Chang Jiang distinguished professor in 2008. He relocated to Shenzhen University in 2013 to found the Nanophotonics Research Centre. His current work deals with optical vortices and applications, optical trapping, plasmonics in microscopy, and optical communication with orbital angular momentum. He has published more than 250 journal papers in optics and photonics. He is a SPIE and OSA Fellow.