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Complete genome sequence of a putative new and distinct caulimovirus from Metaplexis japonica (Thunb.) Makino in China

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Abstract

In 2019, a new disease characterized by yellow mottling on leaves was observed on Metaplexis japonica (Thunb.) Makino plants in Liaoning, China. Using RNA sequencing and PCR with overlapping primers, the complete nucleotide sequence of the circular double-stranded DNA genome of a new virus similar to caulimoviruses in diseased samples was determined. The genome comprised 7733 base pairs and was predicted to encode six proteins. It had the closest relationship to a caulimovirus, strawberry vein banding virus, based on sequence comparisons and phylogenetic analysis. Species-level sequence divergences from caulimoviruses were also observed in the conserved genomic regions. A complete episomal viral genome sequence in symptomatic M. japonica plants was identified, and the name “metaplexis yellow mottle-associated virus” is proposed for this virus.

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Acknowledgments

This work was supported by funds from the Support Program for Young and Middle-Aged Scientific and Technological Innovation Talents of Shenyang City (RC200278). We would like to thank Dr. Gao Fangluan of Fujian Agriculture and Forestry University for useful discussion on the construction of an evolutionary tree. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, 110044, Liaoning, China

    Caixia Yang, Jia Sun, Junzhu Wang, Shunyan Zhang & Meichun Yu

  2. National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Beibei, Chongqing, 400712, China

    Song Zhang & Mengji Cao

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  1. Caixia Yang
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  2. Jia Sun
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  3. Song Zhang
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  4. Junzhu Wang
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  5. Shunyan Zhang
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  6. Meichun Yu
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  7. Mengji Cao
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Correspondence to Caixia Yang or Mengji Cao.

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Yang, C., Sun, J., Zhang, S. et al. Complete genome sequence of a putative new and distinct caulimovirus from Metaplexis japonica (Thunb.) Makino in China. Arch Virol 166, 3433–3436 (2021). https://doi.org/10.1007/s00705-021-05246-0

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  • DOI: https://doi.org/10.1007/s00705-021-05246-0

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