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Combined biostimulation and bioaugmentation for chlorpyrifos degradation in laboratory microcosms

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Abstract

Chlorpyrifos (CP) is a persistent organophosphorus pesticide (OP) used in soil ecosystem for insect control. Bioremediation process has been proven promising in degrading these toxic molecules and restoring the physio-chemical properties of soil. This work reports a laboratory microcosm study in both non-sterile & sterile conditions, conducted over a period of 56 days to examine the combined effect of additional supplements like biostimulants (BSs) such as N, P, and K in the presence of suitable carrier materials (compost, wheat straw, and corncob) along with bioaugmentation by a Ochrobactrum sp. CPD-03 on CP degradation from the contaminated soil. CP degradation was thoroughly monitored at an interval of 7 days over a period of 56 days. Results showed biostimulation and bioaugmentation along with compost as carrier material had shown higher CP degradation efficiency of 76 ± 2.8 and 74 ± 1.6% in non-sterile and sterile microcosms over a period of 56 days. Moreover, bacterial community profiling (16s rRNA and opd gene) demonstrated increased microbial counts, corroborating the efficiency of the bioremediation process. The survival of CPD-03 at the end of the assay validated its ability of colonizing modified soils. By this integrated method with compost as carrier material, bioremediation process could be enhanced for restoration CP-contaminated soils.

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Acknowledgements

We would like to thank Department of Biotechnology (DBT), Govt. of India, New Delhi for the research grant (BT/PR7580/BCE/8/1009/2013). All authors remain highly grateful to the Director, School of Biotechnology, KIIT University for infrastructure facility which had enabled us to work.

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  1. Environmental Biotechnology Laboratory, School of Biotechnology, Kalinga Institute of Industrial Technology-KIIT (Deemed to be University), Bhubaneswar, 751024, Odisha, India

    Tanmaya Nayak, Tapan Kumar Adhya, Mahendra Rakshit & Vishakha Raina

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  1. Tanmaya Nayak
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  2. Tapan Kumar Adhya
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Correspondence to Vishakha Raina.

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Nayak, T., Adhya, T.K., Rakshit, M. et al. Combined biostimulation and bioaugmentation for chlorpyrifos degradation in laboratory microcosms. 3 Biotech 11, 439 (2021). https://doi.org/10.1007/s13205-021-02980-9

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