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Catalase and Superoxide Dismutase Activity in Erythrocytes and the Methemoglobin Level in Blood of the Black Scorpionfish (Scorpaena porcus, Linnaeus 1758) Exposed to Acute Hypoxia

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Abstract

The effect of exposure to acute hypoxia on the level of methemoglobin (MtHb) in blood and the activity of catalase (CAT) and superoxide dismutase (SOD) in erythrocytes of the Black Scorpionfish (Scorpaena porcus, Linnaeus 1758) was studied in experiments in vivo. The O2 concentration in seawater was reduced from 8.5–8.7 mgO2/L to the required values by pumping N2 into the water for 1.5–2.0 h. The concentration range of 0.35–8.50 mgO2/L was studied. The exposure lasted for 4 h at a water temperature of 14–16°C. This showed that at oxygen concentrations in water less than 2 mgO2/L part of hemoglobin passes into the ferric form. The effect was most pronounced in the case of acute hypoxia exposure (less than 1 mgO2/L). The maximum increase in the MtHb level was 19% of the total level of the pigment in blood. This correlated with a significant increase in the SOD and CAT activity in erythrocytes (with the coefficient of determination R2 higher than 0.750) and indicated an increase in the dismutation of superoxide anion in red blood cells.

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Funding

This study was conducted within the framework of the State Program no. AAAA-A18-118021490093-4 and supported in part by the Russian Foundation for Basic Research (project no. 20-04-00037).

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Authors and Affiliations

  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, 299011, Sevastopol, Russia

    A. A. Soldatov, T. I. Andreenko, T. A. Kukhareva, A. Yu. Andreeva & E. S. Kladchenko

  2. Sevastopol State University, 299053, Sevastopol, Russia

    A. A. Soldatov & T. I. Andreenko

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  1. A. A. Soldatov
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  2. T. I. Andreenko
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Correspondence to A. A. Soldatov.

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Translated by E. Shvetsov

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Soldatov, A.A., Andreenko, T.I., Kukhareva, T.A. et al. Catalase and Superoxide Dismutase Activity in Erythrocytes and the Methemoglobin Level in Blood of the Black Scorpionfish (Scorpaena porcus, Linnaeus 1758) Exposed to Acute Hypoxia. Russ J Mar Biol 47, 283–289 (2021). https://doi.org/10.1134/S106307402104012X

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