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Preparation and characterization of microrod hydroxyapatite bundles obtained from oyster shells through microwave irradiation

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Abstract

Hydroxyapatite (HA) is similar to the inorganic component of human bone and teeth. Because of its excellent biocompatibility, bioactivity, and osteoconductivity, HA is widely used as a biomedical material. However, its biodegradability is poor. Oyster shells are mainly composed of CaCO3 and other trace elements, such as Mg, Sr, K, and Na. As a raw material for synthesizing HA, oyster shells not only serve as biowaste for efficient utilization but also contain various trace elements that are beneficial to the growth of bone tissue; they can thus enhance the overall biological performance of the bioceramic. In this study, microrod HA bundles were successfully synthesized using microwave irradiation with the aid of ethylene diamine tetra-acetic acid disodium salt (Na2EDTA) as a chelating agent. The formation of microrod HA bundles was expected to promote the material’s biodegradability, and the bundles could have potential in the field of bone tissue repair. The optimal parameters for synthesis of microrod HA bundles were a Na2EDTA-to-oyster-shell-powder ratio of 2/2 and a microwave power of 700 W. The microrod HA bundles degraded more easily than did the irregular HA aggregates, indicating that the morphology of the microrod bundles resulted in a higher HA degradation rate. Bioactivity tests revealed that numerous spherical apatite particles formed on the surface of the microrod HA bundles, indicating that they had excellent bioactivity. Finally, the microrod HA bundles had high protein adsorption capability, which facilitates the control of cell attachment and proliferation and is thus beneficial to tissue regeneration.

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Acknowledgements

The authors acknowledge the partial financial support of Ministry ofScience and Technology of Taiwan (MOST 108-2813-C-390-014-E).

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Authors and Affiliations

  1. Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan

    Shih-Ching Wu & Hsueh-Chuan Hsu

  2. Department of Life Sciences, National University of Kaohsiung, Kaohsiung, 81148, Taiwan

    Yu-Lin Kao

  3. Department of Chemical and Materials Engineering, National University of Kaohsiung, 700 Kaohsiung University Rd., Nanzih District, Kaohsiung, 81148, Taiwan

    Yi-Cheng Lu & Wen-Fu Ho

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  1. Shih-Ching Wu
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Correspondence to Wen-Fu Ho.

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Shih-Ching Wu and Yu-Lin Kao contributed equally to this work.

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Wu, SC., Kao, YL., Lu, YC. et al. Preparation and characterization of microrod hydroxyapatite bundles obtained from oyster shells through microwave irradiation. J Aust Ceram Soc 57, 1541–1551 (2021). https://doi.org/10.1007/s41779-021-00657-3

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