Abstract
Background
The potential role of HIST1H2BN in prostate cancer remains unclear.
Objective
To evaluate the carcinogenic role of HIST1H2BN in prostate cancer.
Methods
The expression of HIST1H2BN in prostate cancer was analyzed using TCGA database and clinical samples. The roles and mechanisms of HIST1H2BN were investigated in DU145 and PC3 cells.
Results
HIST1H2BN was significantly upregulated in prostate cancer. HIST1H2BN knockdown inhibited cell proliferation, migration and EMT phenotype in prostate cancer cells. Downregulating HIST1H2BN diminished the expression and binding activity of NF-κB p65, then influenced the expression of MMP2 and MMP9.
Conclusion
This is the first study to elaborate a HIST1H2BN-NF-κB-EMT regulatory axis in oncogenesis, indicating that HIST1H2BN might be potential therapeutic target for prostate cancer.
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Acknowledgements
This work was supported by Grants from the National Natural Science Foundation of China (Grant No. 81773240), The Natural Science Foundation of Jiangsu Province (Grant No. BK20181118), Nanjing Medical Science and technique Development Foundation (Grant No. QRX17062) and Csco-Haosen Cancer Research Foundation (Grant No. Y-HS2019/2–028).
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Xiaowei Wei, Luwei Xu, Haiyan Xia, Juan Zhang and Yuhan Chang declare that they have no conflict of interest.
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This study had been approved by the Ethics Committee of Nanjing First Hospital (approval number 2017 − 155). Informed consent was obtained from all individual participants included in the study.
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13258_2021_1164_MOESM1_ESM.tif
Supplementary material 1 Lentivirus-mediated shRNA infection efficiency of DU145 and PC3 cells. A, B, Relative mRNA expression level of HIST1H2BN in DU145 and PC3 cells infected with shCtrl lentivirus and shHIST1H2BN lentivirus( **P < 0.01, ***P < 0.001). C, HIST1H2BN protein in DU145 and PC3 cells infected with shCtrl lentivirus and shHIST1H2BN lentivirus were tested by western blot. D, The relationship between HIST1H2BN and RelA-MMP2/9 signal pathway was evaluated in DU145 cells (TIF 1454.5 kb)
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Zhang, J., Chang, Y., Xia, H. et al. HIST1H2BN induced cell proliferation and EMT phenotype in prostate cancer via NF-κB signal pathway. Genes Genom 43, 1361–1369 (2021). https://doi.org/10.1007/s13258-021-01164-2
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DOI: https://doi.org/10.1007/s13258-021-01164-2