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In vitro embryo production from early antral follicles of goats fed with a whole full-fat linseed based diet

Published online by Cambridge University Press:  17 September 2021

J. Cadenas ,
N.A.R. Sá ,
A.C.A. Ferreira ,
V.M. Paes ,
F.L.N. Aguiar Open the ORCID record for F.L.N. Aguiar [Opens in a new window] ,
C.C.L Fernandes ,
R. Rossetto ,
B.G. Alves ,
G.A. Apgar  and
D.M. Magalhães-Padilha Open the ORCID record for D.M. Magalhães-Padilha [Opens in a new window]
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J. Cadenas
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
N.A.R. Sá
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
A.C.A. Ferreira
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
V.M. Paes
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
F.L.N. Aguiar
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
C.C.L Fernandes
Affiliation:
Laboratory of Ruminant Production and Nutrition (LANUPRUMI), State University of Ceará, Fortaleza, CE, Brazil
R. Rossetto
Affiliation:
Laboratory of Ruminant Production and Nutrition (LANUPRUMI), State University of Ceará, Fortaleza, CE, Brazil
B.G. Alves
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
G.A. Apgar
Affiliation:
Department of Animal Sciences, Food and Nutrition, Southern Illinois University-Carbondale, USA
D.M. Magalhães-Padilha*
Affiliation:
Postgraduate Biotechnology, Potiguar University/Laureate International Universities, Natal, RN, Brazil
D. Rondina
Affiliation:
Laboratory of Ruminant Production and Nutrition (LANUPRUMI), State University of Ceará, Fortaleza, CE, Brazil
J.R. Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil
*
Author for correspondence: Deborah de Melo Magalhães-Padilha. Postgraduate Biotechnology, Potiguar University/Laureate International Universities (UnP) Av. Sen. Salgado Filho, 1610 – Lagoa Nova, Natal – RN – Brasil. CEP: 59056–000. Tel: +55 84 4020 7890. Fax: +55 84 3215 1366. E-mail: dmmvet@hotmail.com
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Summary

The present study aimed to use an in vitro follicle culture (IVFC) biotechnique as a tool to evaluate the influence of whole flaxseed as a feed supplementation in the diet on the in vitro development of caprine early antral follicles (EAFs) and further embryo production. In total, 18 adult goats were homogeneously allocated into two diet groups: Control and Flaxseed. EAFs from both experimental groups (300–400 µm) were isolated and cultured in vitro for 18 days. After IVFC, recovered cumulus–oocyte complexes were submitted to in vitro maturation, and subsequently to IVF and in vitro embryo culture. The endpoints evaluated were follicular growth and morphology, oocyte recovery rate and diameter, sperm penetration, pronuclei formation, embryo development, and estradiol production. The addition of the whole flaxseed in the diet did not affect (P > 0.05) follicular growth and diameter. A higher (P < 0.05) percentage of oocytes ≥ 110 µm was recovered from the flaxseed treatment. However, the sperm penetration rate was higher (P < 0.05) in the control treatment when compared with the flaxseed treatment, but no differences were found regarding the rate of fertilization nor cleaved embryos. In conclusion, dietary flaxseed increased the recovery rate of fully grown oocytes, but it did negatively affect the sperm penetration rate, even though there was no further effect on the cleavage rate.

Keywords

Early antralEmbryosFlaxseedFollicle cultureGoat
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 194 - 199
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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