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Fatty acid-binding protein 5 activates cyclooxygenase-2 and promotes hypoxic injury in LO2 cells

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Abstract

Background

Hepatic hypoxia always results in liver inflammation and dysfunction. Fatty acid-binding protein 5 (FABP5) has been reported as a hypoxia-inducible gene and a regulator of inflammatory reaction.

Objective

To evaluate the effects of FABP5 on hypoxic liver injury and its potential mechanisms.

Results

FABP5 was hypoxia-inducible expression in liver tissues and LO2 cells. The expression levels of IL6 and TNFα were upregulated with the increasing expression of FABP5 in LO2 cells. Downregulation of FABP5 expression decreased the expression levels of ALT, AST and increased cell viability of hypoxic LO2 cells. Downregulation of FABP5 expression inhibited the cyclooxygenase-2/prostaglandin E2 (COX-2/PGE2) pathway and decreased inflammatory reaction in hypoxic LO2 cells.

Conclusions

FABP5 was shown to activate COX-2/PGE2 pathway and promote hypoxic injury in LO2 cells. FABP5 may be a potential target for the treatment of hypoxic liver injury.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No 81702408). The authors thank Pro Rui Liao (Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University) for some helpful advice in article revision.

Funding

This work was supported by the National Natural Science Foundation of China (No 81702408).

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, No 1,Youyi Road, Yuzhong District, Chongqing, 400016, People’s Republic of China

    Long Pan, Heng Xiao, Xin Deng & Qingsong Chen

  2. Urology Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Yihan Pang

Authors
  1. Long Pan
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  2. Yihan Pang
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  3. Heng Xiao
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  4. Xin Deng
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  5. Qingsong Chen
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Contributions

LP: conceptualization, methodology, software, and writing—reviewing and editing YP: data curation and writing—original draft preparation. HX: visualization and investigation. XD: supervision, software, and validation. QC: investigation and software.

Corresponding author

Correspondence to Long Pan.

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Conflict of interest

Long Pan declares that he has no conflict of interest. Yihan Pang declares that she has no conflict of interest. Heng Xiao declares that he has no conflict of interest. Xin Deng declares that he has no conflict of interest. Qingsong Chen declares that he has no conflict of interest.

Ethical approval

All the authors declare that all the experimental procedures were performed in accordance with the ethical standards as recommended by the Institutional Committee of Review Board of the First Affiliated Hospital of Chongqing Medical University.

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Informed consent was obtained from each patient.

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Pan, L., Pang, Y., Xiao, H. et al. Fatty acid-binding protein 5 activates cyclooxygenase-2 and promotes hypoxic injury in LO2 cells. Mol. Cell. Toxicol. 18, 23–30 (2022). https://doi.org/10.1007/s13273-021-00158-1

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  • DOI: https://doi.org/10.1007/s13273-021-00158-1

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