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Paraprobiotic Lacticaseibacillus rhamnosus Protects Intestinal Damage in an Experimental Murine Model of Mucositis

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Abstract

Intestinal mucositis (IM) is a common side effect resulting from cancer treatment. However, the management so far has not been very effective. In the last years, the role of the gut microbiota in the development and severity of mucositis has been studied. Therefore, the use of probiotics and paraprobiotics could have a potential therapeutic effect on IM. The aim of our study was to investigate the impact of the administration of Lacticaseibacillus rhamnosus (L. rhamnosus) CGMCC1.3724 and the paraprobiotic on IM in mice. For 13 days, male Balb/c mice were divided into six groups: control (CTL) and mucositis (MUC)/0.1 mL of saline; CTL LrV and MUC LrV/0.1 mL of 108 CFU of viable Lr; CTL LrI and MUC LrI/0.1 mL of 108 CFU of inactivated Lr. On the 10th day, mice from the MUC, MUC LrV, and MUC LrI groups received an intraperitoneal injection (300 mg/kg) of 5-fluorouracil to induce mucositis. The results showed that the administration of the chemotherapeutic agent increased the weight loss and intestinal permeability of the animals in the MUC and MUC LrV groups. However, administration of paraprobiotic reduced weight loss and maintained PI at physiological levels. The paraprobiotic also preserved the villi and intestinal crypts, reduced the inflammatory infiltrate, and increased the mucus secretion, Muc2 gene expression, and Treg cells frequency.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES awarded the student’s scholarship.

Funding

This work was supported by grants provided by the Conselho Nacional para o Desenvolvimento Cientifico e Tecnológico from Brazil (CNPq—APQ-00593–14; 303506/2019–9; 428548/2018–0), by the Fundação de Amparo a Pesquisa de Minas Gerais (FAPEMIG—APQ-00593–14; PPM-00083–18) and by the Pro Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq/ UFMG).

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Authors and Affiliations

  1. Programa de Pós-Graduação Em Ciência de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Luísa Martins Trindade

  2. Programa de Pós-Graduação Em Bioquímica E Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Lícia Torres & Gregório Cavalcante

  3. Programa de Pós-Graduação Em Nutrição E Saúde, Departamento de Nutrição, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Isabel David Matos, Tatiani Uceli Maioli & Simone de Vasconcelos Generoso

  4. Programa de Pós-Graduação Em Microbiologia, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Vivian Correia Miranda & Flaviano dos Santos Martins

  5. Programa de Pós-Graduação Em Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Luís Cláudio Lima de Jesus, Pamela Mancha-Agresti & Vasco Ariston de Carvalho Azevedo

  6. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Jonathan Júnio de Souza Oliveira & Geovanni Dantas Cassali

  7. Departamento de Análises Clínicas E Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Valbert Nascimento Cardoso

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  1. Luísa Martins Trindade
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Contributions

L.M.T, S.V.G., V.N.C., and F.S.M. designed the work; L.M.T., L.T., I.D.M., V.M.S., L.C.L.J., G.C., J.J.S.O., G.C.D., V.A.C.A., and T.U.M. acquired data; P.M.A, L.M.T., S.V.G., and F.S.M. played an important role in interpreting the results; L.M.T, S.V.G., V.N.C., and F.S.M. drafted or revised the manuscript. All authors have read and approved the final manuscript and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to Simone de Vasconcelos Generoso.

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All procedures involving animals were performed according to the guidelines for care and use of laboratory animals indicated by the Conselho Nacional de Experimentação Animal (CONCEA) for the care and use of laboratory animals and approved by the Comissão de Ética no Uso de Animais of Federal University of Minas Gerais, under protocol number 66/2018 and according to ARRIVE guidelines.

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Trindade, L.M., Torres, L., Matos, I.D. et al. Paraprobiotic Lacticaseibacillus rhamnosus Protects Intestinal Damage in an Experimental Murine Model of Mucositis. Probiotics & Antimicro. Prot. 15, 338–350 (2023). https://doi.org/10.1007/s12602-021-09842-z

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