Abstract
Fructokinase (FRK) is the main fructose phosphorylase and plays an important role in catalyzing the irreversible reaction of free fructose phosphorylation. In order to study the regulatory effect of different forms and concentrations of nitrogen on PtFRK genes in Populus trichocarpa, seven genes encoding the hypothetical FRK proteins were identified in Populus trichocarpa genome by bioinformatics method. Phylogenetic analysis revealed that PtFRK family genes can be divided into two subgroups: SI (PtFRK 1, 3, 4, 6) and SII (PtFRK 2, 5, 7). The tissue-specific expression data obtained from PopGenIE indicate that PtFRK2, 3, 4 and 5 are expressed highly in the stem. Quantitative real-time RT-PCR illustrate that PtFRK1-7 showed different expression patterns in different tissues under different concentrations and morphological nitrogen application. Under high nitrate treatment, the expression levels of PtFRK1, 2, 3 and 6 in stem increased significantly, while under low nitrate treatment, only the expression of PtFRK1, 4 in the upper stem and the expression of PtFRK3, 5 in the lower stem increased significantly. In contrast, ammonium tends to inhibit the expression of PtFRKs in lower stems, the expression levels of PtFRK2, 3, 4 and 5 are significantly reduced under ammonium treatment. However, high ammonium had significant effects on PtFRK6 in the apical bud and upper leaves, which were 6 and 8 times of the control, respectively. These results laid the foundation for the study of the PtFRK gene family of poplar and provided a theoretical basis for the molecular mechanism of nitrogen regulating cell wall development.
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This research was supported by the Fundamental Research Funds for the Central Universities (2572020BA02), Key Project of Natural Science Foundation of Heilongjiang Province of China, Joint guidance project of the Natural Science Foundation of Heilongjiang Province of China, Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team), the National Natural Science Foundation of China (Grant nos. 31600534), the Fundamental Research Funds for the Central Universities (2572019CT02) and the 111 Project (B16010).
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Z.Z. and C.Q. conceived and designed the study, Z.Z. and C.Q. performed most of the experiments, S.Z. and L.C. conducted the sampling, X.S. and J.Y. performed bioinformatics calculations, X.X., Z.X. and G.L. processed and analyzed the data, and Z.Z., C.Q., and G.L. wrote the manuscript.
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Zuo, Z., Sun, X., Cao, L. et al. Genome-wide identification of FRK genes in Populus trichocarpa and their expression under different nitrogen treatments. Physiol Mol Biol Plants 27, 1919–1931 (2021). https://doi.org/10.1007/s12298-021-01055-6
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DOI: https://doi.org/10.1007/s12298-021-01055-6