Abstract
For diagnosis of neuroborreliosis, calculation of the antibody index, based on Euroimmun Anti-Borrelia plus VlsE ELISA was compared to Virotech Borrelia Europe plus TpN17 immunoblot-based detection of Borrelia-specific intrathecal antibody production. CXCL13 results in cerebrospinal fluid were used to evaluate discordant results. A total of 64 serum/CSF pairs were analysed. Patients were classified according to European Federation of Neurological Societies criteria incorporating Virotech results. For the Euroimmun assay, a sensitivity of 100% and specificity of 94% was found. Agreement between the both tests was almost perfect (κ 0.81). Both methods are appropriate for the detection of Borrelia-specific intrathecal antibody production.
Data availability
Due to the retrospective nature of this research, participants of this study did not agree for their data to be shared publicly, so supporting data is not available.
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The study protocol was conducted following good clinical practice guidelines. The study (study number S62243) was approved by the Ethics Committee of the University Hospitals Leuven, Belgium.
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De Bont, E., Lagrou, K. & Depypere, M. Comparison of the Euroimmun Borrelia ‘antibody index’ with Virotech immunoblot-based detection of intrathecal Borrelia antibody production for the diagnosis of Lyme neuroborreliosis. Eur J Clin Microbiol Infect Dis 41, 155–161 (2022). https://doi.org/10.1007/s10096-021-04343-x
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DOI: https://doi.org/10.1007/s10096-021-04343-x