Abstract
Priming of seeds is a simple and low-cost method in agriculture that improves plant growth, crop yield, and manages pathogens. The current study was carried out to determine SA seed priming effect on susceptibility and resistance of two wheat cultivars (Falat and Sumai3) to Fusarium graminearum at the seedling stage. Both the 1 and 2 mM SA-primed seeds showed a higher level of resistance against F. graminearum, as a lower disease incidence of 53% and 63% in the Falat and 48% and 76% in the Sumai3 was observed compared to the F. graminearum-inoculated water-primed control. Along with the increased resistance induced by SA priming, we observed increased activities of superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidases (POX) and phenylalanine ammonia lyase (PAL), in addition to higher mRNA accumulation of PAL, β-1,3-glucanase (GLU-2), chitinase (CHI), cytochrome P450 (CYP), and pleiotropic drug resistance (PDR) genes compared to the water-primed F. graminearum-inoculated control. Therefore, SA seed priming is a suitable alternative to manage the incidence of F. graminearum infection by triggering plant defense responses.
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The authors would like to thank the Iran National Elite Foundation (INEF) for financial support.
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Naser Safaei designed experiments with the assistance of Mona Sorahinobar, Mona Sorahinobar carried out experiments, Mona Sorahinobar and Babak Moradi analyzed experimental results and data and wrote the manuscript.
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12374_2021_9329_MOESM1_ESM.jpg
Supplementary file1 (JPG 1721 KB) Trypan blue staining of wheat seedlings, 7 dai with F. graminearum confirming pathogen penetration in crown tissue
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Supplementary file2 (JPG 6863 KB) Wheat seedlings originated from SA primed seeds displaying increased resistance to F. graminearum infection with lower brown or black lesion in crown and better root growth
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Sorahinobar, M., Safaie, N. & Moradi, B. Salicylic Acid Seed Priming Enhanced Resistance in Wheat Against Fusarium graminearum Seedling Blight. J. Plant Biol. 65, 423–434 (2022). https://doi.org/10.1007/s12374-021-09329-y
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DOI: https://doi.org/10.1007/s12374-021-09329-y