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VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism

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Abstract

Potato virus Y (PVY) is one of the most common and harmful plant viruses. Translation of viral RNA starts with the interaction between the plant cap-binding translation initiation factors eIF4E and viral genome-linked protein (VPg) covalently attached to the viral RNA. Disruption of this interaction is one of the natural mechanisms of plant resistance to PVY. The multigene eIF4E family in the potato (Solanum tuberosum L.) genome contains genes for the translation initiation factors eIF4E1, eIF4E2, and eIF(iso)4E. However, which of these factors can be recruited by the PVY, as well as the mechanism of this interaction, remain obscure. Here, we showed that the most common VPg variant from the PVY strain NTN interacts with eIF4E1 and eIF4E2, but not with eIF(iso)4E. Based on the VPg, eIF4E1, and eIF4E2 models and data on the natural polymorphism of VPg amino acid sequence, we suggested that the key role in the recognition of potato cap-binding factors belongs to the R104 residue of VPg. To verify this hypothesis, we created VPg mutants with substitutions at position 104 and examined their ability to interact with potato eIF4E factors. The obtained data were used to build the theoretical model of the VPg-eIF4E2 complex that differs significantly from the earlier models of VPg complexes with eIF4E proteins, but is in a good agreement with the current biochemical data.

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Acknowledgments

The authors are grateful to D. G. Kozlov (Kurchatov Institute Research Center) and I. I. Gubaidullin (Kurchatov Institute Research Center, Genetics Research Institute, Kurchatov Genomic Center) for valuable advice on yeast cultivation and to Dr. J.-L. Gallois (INRA) for kindly providing the yeast Jo55 strain for complementation analysis. The work was performed using the scientific equipment of the Center for Collective Use “Biotechnology” at the All-Russia Institute of Agricultural Biotechnology (Moscow, Russia; agreement RFMEFI62114×0003).

Funding

This research was supported by the Russian Foundation for Basic Research (project no. 17-29-08024 ofi_m). Western blot analysis was performed due to support of the State task no. AAAA-A19-119092390041-5.

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Authors and Affiliations

  1. All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550, Moscow, Russia

    Marina V. Lebedeva, Vasiliy V. Taranov & Alexey V. Babakov

  2. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    Ekaterina Y. Nikonova & Oleg S. Nikonov

  3. Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432, Chernogolovka, Moscow Region, Russia

    Alexey A. Terentiev

  4. Scientific and Educational Center in Chernogolovka, Moscow Region State University, 141014, Mytishchi, Moscow Region, Russia

    Alexey A. Terentiev

  5. Faculty of Fundamental Physical and Chemical Engineering, Lomonosov Moscow State University, 119991, Moscow, Russia

    Alexey A. Terentiev

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  1. Marina V. Lebedeva
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Correspondence to Marina V. Lebedeva.

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Lebedeva, M.V., Nikonova, E.Y., Terentiev, A.A. et al. VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism. Biochemistry Moscow 86, 1128–1138 (2021). https://doi.org/10.1134/S000629792109008X

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