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Comparison of the Bulk and Rhizosphere Soil Prokaryotic Communities Between Wild and Reintroduced Manglietiastrum sinicum Plants, a Threatened Species with Extremely Small Populations

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Abstract

Huagaimu (Manglietiastrum sinicum) trees are critically endangered species and classified as a plant species with extremely small populations in China. Rhizospheres and bulk soils prokaryotic communities play an important role to protect and promote plants health and growth. However, the compositions and structures of prokaryotic communities in wild and reintroduced M. sinicum rhizospheres and bulk soils are still poorly understood. In the present study, prokaryotic communities in wild and reintroduced M. sinicum rhizospheres and bulk soils were compared using high-throughput sequencing. Thirty-two phyla, 76 classes, 193 orders, 296 families, and 470 genera of prokaryotes were obtained. Proteobacteria and Acidobacteria were the two most abundant phyla in all soil samples. The compositions and structures of prokaryotic communities were overall similar, and the abundance of some taxa varied significantly among soil samples. Soil prokaryotic communities were significantly affected by soil pH, total nitrogen, total phosphorus, and total potassium. Eleven of predicted functions were significantly different among the four soil groups. This study provides for the first insights into the compositions, structures, and potential functions of prokaryotic communities associated with wild and reintroduced M. sinicum rhizospheres and bulk soils, and providing a foundation for future research to help protect this endangered species.

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Acknowledgements

We would like to thanks the National Natural Science Foundation of China (NSFC) and the Joint Project of Basic Research of Yunnan Provincial Universities for grants support.

Funding

This work was supported by grants from the National Natural Science Foundation of China (31960220, 31660089, 32071570, 31660001, and 31760017) and the Joint Project of Basic Research of Yunnan Provincial Universities (2018FH001028).

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Author notes

  1. Dai-Fa Su and Qing-Qing Shen have equally contributed to this work.

Authors and Affiliations

  1. School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Dai-Fa Su, Qing-Qing Shen, Jun-Yu Yang, Zhi-Ying Li, Wei Xiao, Yong-Xia Wang, Zhang-Gui Ding & Xiao-Long Cui

  2. School of Sanqi Medicine, Wenshan University, Wenshan, 663099, Yunnan, People’s Republic of China

    Qing-Qing Shen

  3. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Dai-Fa Su, Jun-Yu Yang & Xiao-Long Cui

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Contributions

The experiments were conceived and designed by D-FS, Q-QS, J-YY, Z-YL, WX, Y-XW, Z-GD, and X-LC; the experiments were performed by D-FS, Q-QS, J-YY, Z-YL, WX, Y-XW, Z-GD, and X-LC; the data were analyzed by D-FS, Q-QS, and J-YY; the manuscript was written by D-FS and Q-QS. All authors read and improved the manuscript and agreed to the published version of the manuscript.

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Correspondence to Xiao-Long Cui.

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Su, DF., Shen, QQ., Yang, JY. et al. Comparison of the Bulk and Rhizosphere Soil Prokaryotic Communities Between Wild and Reintroduced Manglietiastrum sinicum Plants, a Threatened Species with Extremely Small Populations. Curr Microbiol 78, 3877–3890 (2021). https://doi.org/10.1007/s00284-021-02653-z

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