Issue 19, 2021
From the journal:

Lab on a Chip

Review: tomographic imaging flow cytometry

Andreas Kleiber,†a   Daniel Kraus, ORCID logo †*a   Thomas Henkel ORCID logo a  and  Wolfgang Fritzsche ORCID logo a  

* Corresponding authors

a Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, D-07745 Jena, Germany

Abstract

Within the last decades, conventional flow cytometry (FC) has evolved as a powerful measurement method in clinical diagnostics, biology, life sciences and healthcare. Imaging flow cytometry (IFC) extends the power of traditional FC by adding high resolution optical and spectroscopic information. However, the conventional IFC only provides a 2D projection of a 3D object. To overcome this limitation, tomographic imaging flow cytometry (tIFC) was developed to access 3D information about the target particles. The goal of tIFC is to visualize surfaces and internal structures in a holistic way. This review article gives an overview of the past and current developments in tIFC.

Graphical abstract: Review: tomographic imaging flow cytometry

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Article information

DOI
https://doi.org/10.1039/D1LC00533B
Article type
Critical Review
Submitted
16 Jun 2021
Accepted
18 Aug 2021
First published
13 Sep 2021
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2021,21, 3655-3666

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Review: tomographic imaging flow cytometry

A. Kleiber, D. Kraus, T. Henkel and W. Fritzsche, Lab Chip, 2021, 21, 3655 DOI: 10.1039/D1LC00533B

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