Chitin deacetylase 2 is essential for molting and survival of Tetranychus urticae
Graphical abstract
Section snippets
Mites
The population of an insecticide-susceptible strain of T. urticae (red form) is raised at 26 °C, 45% relative humidity, and with a of 16:8 h (light: dark) photoperiod on cowpea leaves at Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, under pesticide-free conditions for over 10 years. These mites are assayed each year for high sensitivity to pesticides using the glass slide-dipping method according to FAO standards (Bu et al., 2015a).
Sequence analysis of TuCDA1 and TuCDA2
Sequences of TuCDA1
Sequence analysis of TuCDA1 and TuCDA2
The exon-intron organization of TuCDA1 and TuCDA2 was determined by comparing the TuCDA1 and TuCDA2 sequences with the T. urticae genomic sequences (Fig. 1A). In the T. urticae genome, TuCDA1 was located in scaffold 11, whereas TuCDA2 was located in scaffold 5. TuCDA1 and TuCDA2 both had four exons, however their exon-intron organization were somewhat diverse.
TuCDA1 and TuCDA2 had quite different amino acid sequences with only 76.5% identity. Similar to the insect orthologues, TuCDA1 and TuCDA2
Discussion
Here, the roles of TuCDA1 and TuCDA2 of T. urticae during molting process and mite development were explored. TuCDA1 and TuCDA2 differ in exon-intron organization and amino acid sequence, although TuCDA1 and TuCDA2 have similar domain organization, hydropathy profiles (Xiangshun et al., 2019). Seven crystal structures of CDAs, including one from insects, six from fungi and bacteria, have been determined (Blair et al., 2006; Blair et al., 2005; Blair and Van Aalten, 2004; Liu et al., 2019).
Acknowledgments
This study was financially supported by the Beijing Natural Science Foundation (no. 6212004), the National Natural Science Foundation of China (no. 31670648). We thank all members of Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, for their advice, assistance, and technical help.
References (42)
- et al.
Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum
Insect Biochem. Mol. Biol.
(2008) - et al.
Analysis of functions of the chitin deacetylase gene family in Tribolium castaneum
Insect Biochem. Mol. Biol.
(2009) - et al.
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine
FEBS Lett.
(2004) - et al.
Novel and selective acetylcholinesterase inhibitors for Tetranychus cinnabarinus (Acari: Tetranychidae)
Insect Biochem. Mol. Biol.
(2015) - et al.
Domain organization and phylogenetic analysis of proteins from the chitin deacetylase gene family of Tribolium castaneum and three other species of insects
Insect Biochem. Mol. Biol.
(2008) - et al.
Chapter six - insect chitin: Metabolism, genomics and pest management
- et al.
Insect Chitinases: molecular biology and potential use as biopesticides
Insect Biochem. Mol. Biol.
(1997) - et al.
Structural and biochemical insights into the catalytic mechanisms of two insect chitin deacetylases of the carbohydrate esterase 4 family
J. Biol. Chem.
(2019) The Wigglesworth lecture: insects for studying fundamental problems in biology
J. Insect Physiol.
(2001)- et al.
Serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila
Curr. Biol.
(2006)
Serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila
Curr. Biol.
Group I chitin deacetylases are essential for higher order organization of chitin fibers in beetle cuticle
J. Biol. Chem.
Drosophila Chitinase 2 is expressed in chitin producing organs for cuticle formation
Arthropod. Struct. Dev.
Characterization of a spruce budworm chitin deacetylase gene: stage- and tissue-specific expression, and inhibition using RNA interference
Insect Biochem. Mol. Biol.
RNAi targeting ecdysone receptor blocks the larva to adult development of Tetranychus cinnabarinus
Pestic. Biochem. Physiol.
Chitin deacetylases: new, versatile tools in biotechnology
Trends Biotechnol.
Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: a review
Insect Biochem. Mol. Biol.
Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila Trachea
Curr. Biol.
Helicoidal Organization of Chitin in the cuticle of the migratory locust requires the function of the chitin Deacetylase2 enzyme (LmCDA2)
J. Biol. Chem.
The putative chitin deacetylases serpentine and vermiform have non-redundant functions during Drosophila wing development
Insect Biochem. Mol. Biol.
Evidence for synonymy between Tetranychus urticae and Tetranychus cinnabarinus (Acari, Prostigmata, Tetranychidae): review and new data
Acarologia
Cited by (3)
Alternative splicing of chitin deacetylase 2 regulates chitin and fatty acid metabolism in Asian citrus psyllid, Diaphorina citri
2023, Archives of Insect Biochemistry and Physiology