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Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity

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Abstract

Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peritoneal cavity cell response to in vitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon in vitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory priming by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release in vitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.

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Funding

This work was supported by Ministry of Education, Science and Technological Development Republic of Serbia (contract number 451–03-68/2020–14/200177).

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Author notes

  1.     Stanislava Stanojević and Veljko Blagojević equally contributed to this work.

Authors and Affiliations

  1. Immunology Research Centre “Branislav Janković, Institute of Virology, Vaccines and Sera “Torlak, Belgrade, Serbia

    Stanislava Stanojević, Veljko Blagojević & Ivana Ćuruvija

  2. Department of Chemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Vesna Vujić

  3. Immunology Research Centre “Branislav Janković, Institute of Virology, Vaccines and Sera “Torlak, Belgrade, Serbia

    Stanislava Stanojević

Authors
  1. Stanislava Stanojević
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  2. Veljko Blagojević
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  3. Ivana Ćuruvija
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  4. Vesna Vujić
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Veljko Blagojević, Ivana Ćuruvija, and Vesna Vujić, and study was supervised by Stanislava Stanojević. The first draft of the manuscript was written by Stanislava Stanojević and Veljko Blagojević, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Stanislava Stanojević.

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The experimental protocol and the procedures involving animals and their care were approved by Ministry of Agriculture and Environmental Protection (licence number 323–07-01577/2016–05/14, issued on 02–25-2016), and were in accordance with the principles declared in Directive 2010/63/EU of the European Parliament and of the Council from 22 September 2010 on the protection of animals used for scientific purposes (revising Di.rective 86/609/EEC).

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Stanojević, S., Blagojević, V., Ćuruvija, I. et al. Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. Inflammation 44, 2429–2447 (2021). https://doi.org/10.1007/s10753-021-01513-z

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