Skip to main content
Log in

Construction of an infectious dahlia common mosaic virus clone

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Dahlia is a major ornamental plant that is cultivated worldwide. However, dahlia plants, which are mainly propagated through vegetative reproduction, are susceptible to widespread damage by viruses, and viral control requires that the nature of the infecting virus(es) be known. In this study, dahlia common mosaic virus (DCMV) was detected for the first time in Japan and sequenced. This is the first report of an infectious DCMV clone being constructed, and it will aid in the characterization of DCMV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Availability of data and material

The dahlia common mosaic virus genome sequence has been deposited in the DDBJ/ENA/GenBank database under accession number LC625373.

References

  1. Eid S, Saar DE, Druffel KL, Pappu HR (2011) Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges. Plant Pathol 60:378–383. https://doi.org/10.1111/j.1365-3059.2010.02367.x

    Article  CAS  Google Scholar 

  2. Hadfield J, Linderme D, Shepherd DN, Bezuidenhout M, Lefeuvre P, Martin DP, Varsani A (2011) Complete genome sequence of a dahlia common mosaic virus isolate from New Zealand. Arch Virol 156:2297–2301. https://doi.org/10.1007/s00705-011-1112-y

    Article  CAS  PubMed  Google Scholar 

  3. Pappu HR, Wyatt SD, Druffel KL (2005) Dahlia mosaic virus: Molecular detection and distribution in dahlia in the United States. HortScience 40:697–699. https://doi.org/10.21273/HORTSCI.40.3.697

    Article  Google Scholar 

  4. Sohrab SS (2018) Molecular diagnosis of begomovirus associated with leaf curl disease of Dahlia pinnata. Nov Res Microbiol J 2:114–121. https://doi.org/10.21608/nrmj.2018.22704

    Article  Google Scholar 

  5. International Committee on Taxonomy of Viruses (2020) Genus: Caulimoviridae. https://talk.ictvonline.org/ictv-reports/ictv_online_report/reverse-transcribing-dna-and-rna-viruses/w/caulimoviridae/1362/genus-caulimovirus Accessed 12 May 2021

  6. Brunt AA (1971) Some hosts and properties of dahlia mosaic virus. Ann Appl Biol 67:357–368. https://doi.org/10.1111/j.1744-7348.1971.tb02937.x

    Article  Google Scholar 

  7. Pappu HR, Druffel KL, Miglino R, Van Schadewijk AR (2008) Nucleotide sequence and genome organization of a member of a new and distinct Caulimovirus species from dahlia. Arch Virol 153:2145–2148. https://doi.org/10.1007/s00705-008-0235-2

    Article  CAS  PubMed  Google Scholar 

  8. Chao HY, Chen YK (2012) Molecular characterization of a dahlia mosaic-associated Caulimovirus. Plant Pathol Bull 21:101–114

    CAS  Google Scholar 

  9. Eid S, Pappu HR (2014) Biological studies of three caulimoviruses associated with dahlia (Dahlia variabilis). Can J Plant Pathol 36:110–115. https://doi.org/10.1080/07060661.2013.871753

    Article  Google Scholar 

  10. Asano S, Matsushita Y, Hirayama Y, Naka T (2015) Simultaneous detection of Tomato spotted wilt virus, Dahlia mosaic virus and Chrysanthemum stunt viroid by multiplex RT-PCR in dahlias and their distribution in Japanese dahlias. Lett Appl Microbiol 61:113–120. https://doi.org/10.1111/lam.12442

    Article  CAS  PubMed  Google Scholar 

  11. Martin M (2011) Cutadapt removes adapter sequences from high–throughput sequencing reads. EMBnet 17:10–12. https://doi.org/10.14806/ej.17.1.200

    Article  Google Scholar 

  12. Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. https://doi.org/10.1089/cmb.2012.0021

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Pahalawatta V, Druffel K, Pappu H (2008) A new and distinct species in the genus Caulimovirus exists as an endogenous plant pararetroviral sequence in its host, Dahlia variabilis. Virology 376:253–257. https://doi.org/10.1016/j.virol.2008.03.003

    Article  CAS  PubMed  Google Scholar 

  14. Pahalawatta V, Miglino R, Druffel KB, Jodlowska A, van Schadewijk AR, Pappu HR (2007) Incidence and relative prevalence of distinct caulimoviruses (genus Caulimovirus, family Caulimoviridae) associated with dahlia mosaic in Dahlia variabilis. Plant Dis 91:1194–1197. https://doi.org/10.1094/PDIS-91-9-1194

    Article  CAS  PubMed  Google Scholar 

  15. Delseny M, Hull R (1983) Isolation and characterization of faithful and altered clones of the genomes of cauliflower mosaic virus isolates Cabb B-JI, CM4-184, and Bari I. Plasmid 9:31–41. https://doi.org/10.1016/0147-619X(83)90029-X

    Article  CAS  PubMed  Google Scholar 

  16. Feng M, Zhang H, Pan Y et al (2016) Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone. Virol J 13:164. https://doi.org/10.1186/s12985-016-0624-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Mahmoudpour A (2003) Infectivity of recombinant strawberry vein banding virus DNA. J Gen Virol 84:1377–1381. https://doi.org/10.1099/vir.0.18994-0

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Mr. Akira Seto (Utsunomiya University) for taking photos, and Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.

Funding

This study was supported by a grant-in-aid for early-career scientists, Japan Society for the Promotion of Sciences (grant number 19K15839), to YN.

Author information

Authors and Affiliations

Authors

Contributions

Material preparation, data collection and analysis were performed by DY, YN, and TS. The first draft of the manuscript was written by DY and YN. HN and TN made comments and improved the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Yutaro Neriya.

Ethics declarations

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Additional information

Handling Editor: Elvira Fiallo-Olivé

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Nucleotide sequence data reported are available in the DDBJ/ENA/GenBank databases under the accession number LC625373 (DCMV-JP).

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (XLSX 12 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yamamoto, D., Neriya, Y., Suzuki, T. et al. Construction of an infectious dahlia common mosaic virus clone. Arch Virol 166, 3179–3182 (2021). https://doi.org/10.1007/s00705-021-05225-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-021-05225-5

Navigation