SCHEDULED MAINTENANCE
Image fusion of IR and optical microscopy for mapping of biomolecules in tissue
Asterios
Triantafyllou, 
b
Caroline I.
Smith,
a
Philip J.
Gunning,
c
Peter
Gardner,
d
Janet M.
Risk,
c
Peter
Weightman
a
and
Steve D.
Barrett
*a
b
Caroline I.
Smith,
a
Philip J.
Gunning,
c
Peter
Gardner,
d
Janet M.
Risk,
c
Peter
Weightman
a
and
Steve D.
Barrett
*a
Abstract
It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers.
Please wait while we load your content...
