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The decolorization and degradation of azo dyes by two Stenotrophomonas strains isolated from textile effluent (Tepetitla, Mexico)

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Stenotrophomonas’ metabolic versatility plays important roles in the remediation of contaminated environment and plant growth promotion. We investigated two Stenotrophomonas strains isolated from textile polluted sewage for their ability to decolorize and degrade azo dyes. Two Stenotrophomonas strains (TepeL and TepeS) were isolated from textile effluents (Tepetitla, Mexico) using the selective agar Stenotrophomonas vancomycin, imipenem, amphotericin B agar (SVIA). Isolates’ identity was determined by the sequencing of their partial 16S rRNA fragments. Their abilities to decolorize dyes were tested in a Luria broth supplemented with varying concentrations (50 mg/L–1 g/L) of textile dyes (acidic red, methyl orange, reactive green, acidic yellow, and reactive black). Fourier-transform infrared (FTIR) spectroscopy and ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) metabolite analyses were used to determine the effect of the isolates’ growth on the dyes (acidic red, methyl orange). We also identified the enzymes that may be involved in the degradation process. Phylogenetic analysis based on the 16S rDNA sequences showed that the isolates belong to the genus Stenotrophomonas. Stenotrophomonas sp. TepeL and TepeS respectively decolorize all the azo dyes at the tested concentration except at 1 g/L and degraded the azo dyes. The degradation resulted in the formation of N, N-dimethyl p-phenylenediamine, and sodium 4-amino-1-naphthalenesulfonate from methyl orange and acid red. TepeL and TepeS rapidly decolorized and degraded the azo dyes tested. This result showed that the two isolates have a good potential for the decontamination of textile effluents.

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Acknowledgements

The authors want to express their sincere appreciation to Dra Patricia Claudia Larralde Corona and Dr. Jose Alberto Narvaez Zapata of the Laboratorio de Biotecnologia Industrial for giving us the opportunity to use their sonicator.

Funding

The authors want to acknowledge CONACYT for providing the financial support for the study.

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Authors and Affiliations

  1. Laboratorio de Biotecnología Genómica, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas, Mexico

    José Antonio Vilchis-Carmona, Isabel Cristina Rodríguez-Luna, Temidayo Oluyomi Elufisan, Alejandro Sánchez-Varela & Xianwu Guo

  2. Centro De Investigación En Biotecnología Aplicada, Instituto Politécnico Nacional, Tepetitla, Tlaxcala, Mexico

    Martha Bibbins-Martínez  & Miguel Angel Villalobos-López

  3. Laboratorio de Biotecnología Farmaceútica, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, Mexico

    Gildardo Rivera & Alma D. Paz-Gonzalez

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  1. José Antonio Vilchis-Carmona
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  2. Isabel Cristina Rodríguez-Luna
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Vilchis-Carmona, J.A., Rodríguez-Luna, I.C., Elufisan, T.O. et al. The decolorization and degradation of azo dyes by two Stenotrophomonas strains isolated from textile effluent (Tepetitla, Mexico). Braz J Microbiol 52, 1755–1767 (2021). https://doi.org/10.1007/s42770-021-00542-y

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