Skip to main content
SpringerLink
Log in

Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Fusarium oxysporum

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Fusarium oxysporum is a cosmopolitan plant pathogen that causes fusarium wilt and fusarium root rot in many economically important crops. There is still limited information about mycoviruses that infect F. oxysporum. Here, a novel mitovirus tentatively named "Fusarium oxysporum mitovirus 1" (FoMV1) was identified in F. oxysporum strain B2-10. The genome of FoMV1 is 2,453 nt in length with a predicted AU content of 71.6% and contains one large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF putatively encodes an RNA-dependent RNA polymerase (RdRp) of 723 aa with a molecular mass of 84.98 kDa. The RdRp domain of FoMV1 shares 29.01% to 68.43% sequence identity with the members of the family Mitoviridae. Phylogenetic analysis further suggested that FoMV1 is a new member of a distinct species in the genus Mitovirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Availability of data and material

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Michielse CB, Rep M (2009) Pathogen profile update: Fusarium oxysporum. Mol Plant Pathol 10:311–324. https://doi.org/10.1111/j.1364-3703.2009.00538.x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Pietro AD, Madrid MP, Caracuel Z, Delgado-Jarana J, Roncero MIG (2003) Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Mol Plant Pathol 4:315–325. https://doi.org/10.1046/j.1364-3703.2003.00180.x

    Article  Google Scholar 

  3. Armstrong GM, Armstrong JK (1968) Formae specials and races of Fusarium oxysporum causing a tracheomycosis in the syndrome diseases. Phytopathology 58:1242–1246. https://doi.org/10.1094/phyto-68-19

    Article  Google Scholar 

  4. Chehri K, Salleh B, Zakaria L (2015) Morphological and phylogenetic analysis of Fusarium solani species complex in Malaysia. Microbial Ecol 69:457–471. https://doi.org/10.1007/s00248-014-0494-2

    Article  Google Scholar 

  5. Yang M, Cao JD, Zheng YX, Zhou HF, He MD, Duan J, Tong WJ, Deng XP, Chen XL (2020) First report of fusarium root rot of tobacco caused by Fusarium solani in Lincang, China. Plant Dis 104:1541. https://doi.org/10.1094/PDIS-08-19-1711-PDN

    Article  Google Scholar 

  6. LaMondia JA (2015) Fusarium wilt of tobacco. Crop Protec 73:73–77. https://doi.org/10.1016/j.cropro.2015.03.003

    Article  Google Scholar 

  7. Snow D, Lucas GB (1966) Diseases of Tobacco. J Appl Ecol 3:409. https://doi.org/10.2307/2401265

    Article  Google Scholar 

  8. Ghabrial S, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384. https://doi.org/10.1146/annurev-phyto-080508-081932

    Article  CAS  PubMed  Google Scholar 

  9. Kotta-Loizou I, Coutts RHA (2017) Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLoS Pathog 13:e1006183. https://doi.org/10.1371/journal.ppat.1006183

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Son M, Yu J, Kim KH (2015) Five questions about mycoviruses. PLoS Pathog. https://doi.org/10.1371/journal.ppat.1005172

    Article  PubMed  PubMed Central  Google Scholar 

  11. Herrero N, Dueñas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530. https://doi.org/10.1128/AEM.01954-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Ghabrial SA, Caston JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368. https://doi.org/10.1016/j.virol.2015.02.034

    Article  CAS  PubMed  Google Scholar 

  13. Deng F, Xu R, Boland GJ (2003) Hypovirulence-associated double-stranded RNA from Sclerotinia homoeocarpa is conspecific with Ophiostoma novo-ulmi mitovirus 3a-Ld. Phytopathology 93:1407–1414. https://doi.org/10.1094/PHYTO.2003.93.11.1407

    Article  CAS  PubMed  Google Scholar 

  14. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68. https://doi.org/10.1146/annurev-phyto-102313-050222

    Article  CAS  PubMed  Google Scholar 

  15. Hillman BI, Suzuki N (2004) Viruses of the chestnut blight fungus, Cryphonectria parasitica. Adv Virus Res 63:423–472. https://doi.org/10.1016/S0065-3527(04)63007-7

    Article  CAS  PubMed  Google Scholar 

  16. Xiao Y, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392. https://doi.org/10.1016/S0065-3527(04)63007-7

    Article  Google Scholar 

  17. Xiao Y, Li B, Fu Y, Xie J, Cheng J, Ghabrial SA et al (2013) Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc Natl Acad Sci USA 110(4):1452–1457. https://doi.org/10.1073/pnas.1213755110

    Article  Google Scholar 

  18. Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812. https://doi.org/10.1128/JVI.01830-09

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Salaipeth L, Eusebio-Cope A, Chiba S, Kanematsu S, Suzuki N (2014) Biological properties and expression strategy of Rosellinia necatrix megabirnavirus 1 in an experimental host Cryphonectria parasitica. J Virol 95:740–750. https://doi.org/10.1099/vir.0.058164-0

    Article  CAS  Google Scholar 

  20. Kilic O, Griffin GJ (1998) Effect of dsRNA-containing and dsRNA-free hypovirulent isolates of Fusarium oxysporum on severity of fusarium seedling disease of soybean in naturally infested soil. Plant Soil 201:125–135. https://doi.org/10.1023/A:1004319614390

    Article  CAS  Google Scholar 

  21. Lemus-Minor CG, Carmen CM, García-Pedrajas MD, Pérez-Artés E (2019) Horizontal and vertical transmission of the hypovirulence-associated mycovirus Fusarium oxysporum f. sp. dianthi virus 1. Eur J Plant Pathol 153:645–650. https://doi.org/10.1007/s10658-018-1554-0

    Article  Google Scholar 

  22. Zhao Y, Zhang Y, Wan X, She Y, Li M, Xi H, Xie J, Wen C (2020) A Novel Ourmia-Like Mycovirus Confers Hypovirulence-Associated Traits on Fusarium oxysporum. Front Microbiol. https://doi.org/10.3389/fmicb.2020.569869

    Article  PubMed  PubMed Central  Google Scholar 

  23. Torres-Trenas A, Cañizares MC, García-Pedrajas MD, Pérez-Artés E (2020) Molecular and biological characterization of the first hypovirus identified in fusarium oxysporum. Front Microbiol 10:3131–3143. https://doi.org/10.3389/fmicb.2019.03131

    Article  PubMed  PubMed Central  Google Scholar 

  24. Torres-Trenas A, Pérez-Artés E (2020) Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum. Viruses 12:279–293. https://doi.org/10.3390/v12030279

    Article  CAS  PubMed Central  Google Scholar 

  25. Sato Y, Shamsi W, Jamal A, Bhatti MF, Suzuki N (2020) Hadaka virus 1: a capsidless eleven-segmented positive-sense single-stranded rna virus from a phytopathogenic fungus Fusarium oxysporum. mBio 11:20–38. https://doi.org/10.1128/mBio.00450-20

    Article  Google Scholar 

  26. Mishra PK, Fox RT, Culham A (2003) Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiol Lett 218:329–332. https://doi.org/10.1016/S0378-1097(02)01174-6

    Article  CAS  PubMed  Google Scholar 

  27. O’Donnell K, Sutton DA, Rinaldi MG, Sarver BA, Balajee SA, Schroers HJ et al (2010) Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 48:3708–3718

    Article  CAS  Google Scholar 

  28. Wang J, Ni Y, Liu X, Zhao H, Xiao Y, Xiao X, Li S, Liu H (2020) Divergent RNA Viruses In Macrophomina phaseolina exhibit potential as virocontrol agents. Virus Evolution. https://doi.org/10.1093/ve/veaa095

    Article  PubMed  PubMed Central  Google Scholar 

  29. Yao Z, Zou C, Peng N, Zhu Y, Bao Y, Zhou Q, Wu Q, Chen B, Zhang M (2020) Virome Identification and Characterization of Fusarium sacchari and F. andiyazi: Causative Agents of Pokkah Boeng Disease in Sugarcane. Front Microbiol 11:240. https://doi.org/10.3389/fmicb.2020.00240

    Article  PubMed  PubMed Central  Google Scholar 

  30. Paquin B, Laforest MJ, Forget L, Roewer I, Wang Z, Longcore J, Lang BF (1997) The fungal mitochondrial genome project: Evolution of fungal mitochondrial genomes and their gene expression. Curr Genet 31:380–395. https://doi.org/10.1248/yakushi.126.1179

    Article  CAS  PubMed  Google Scholar 

  31. Nibert ML (2017) Mitovirus UGA (Trp) codon usage parallels that of host mitochondria. Virology 507:96–100. https://doi.org/10.1016/j.virol.2017.04.010

    Article  CAS  PubMed  Google Scholar 

  32. Hong Y, Dover SL, Cole TE, Brasier CM, Buck KW (1999) Multiple mitochondrial viruses in an isolate of the Dutch elm disease fungus Ophiostoma novo-ulmi. Virology 258:118–127. https://doi.org/10.1006/viro.1999.9691

    Article  CAS  PubMed  Google Scholar 

  33. Hillman BI, Cai G (2013) The family Narnaviridae: simplest of RNA viruses. Adv Virus Res 86:149–176. https://doi.org/10.1016/B978-0-12-394315-6.00006-4

    Article  PubMed  Google Scholar 

  34. Wu M, Zhang L, Li G, Jiang D, Ghabrial SA (2010) Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus botrytis cinerea. Virology 406(1):117–126. https://doi.org/10.1016/j.virol.2010.07.010

    Article  CAS  PubMed  Google Scholar 

  35. Huang S, Ghabrial SA (1996) Organization and expression of the double-stranded RNA genome of Helminthosporium victoriae 190S virus, a totivirus infecting a plant pathogenic filamentous fungus. Proc Natl Acad Sci U S A 93:12541–12546. https://doi.org/10.2307/40654

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Wang J, Xiao Y, Liu X, Ni Y, Zhao H, Zhao X, Liu H, Xiao X (2020) Complete genome sequence of a novel victorivirus isolated from the sesame charcoal rot fungus Macrophomina phaseolina. Arch Virol 165(2):509–514. https://doi.org/10.1007/s00705-019-04497-2

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This research work was supported by the Science and Technology Project of Henan Provincial Tobacco Company (2020410000270012). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author information

Authors and Affiliations

  1. Key Laboratory for Green Preservation and Control of Tobacco Diseases and Pest in Huanghuai Growing Area, Institute of Tobacco, Henan Academy of Agricultural Sciences, No. 116, Garden road, Jingshui District, Zhengzhou, 450002, Henan Province, People’s Republic of China

    Jing Wang, Chengjun Li, Rui Qiu, Xiaojie Li, Jun Zhao, Jingke Bai, Yuguo Chen & Shujun Li

Authors
  1. Jing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chengjun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rui Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaojie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jun Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jingke Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yuguo Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shujun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.W. and S.J.L. conceived and designed the experiments; J.W. performed the experiments; R.Q., C.J.L., X.J.L., J.Z., J.K.B., and Y.G.C. collected the samples; J.W. wrote the paper; and S.J.L. and R.Q. revised the paper.

Corresponding author

Correspondence to Shujun Li.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Ioly Kotta-Loizou.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Li, C., Qiu, R. et al. Complete genome sequence of a novel mitovirus from the phytopathogenic fungus Fusarium oxysporum. Arch Virol 166, 3211–3216 (2021). https://doi.org/10.1007/s00705-021-05210-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-021-05210-y

Search

Navigation