Abstract
Olive (O. europaea L.) is an expanding crop in the south of Brazil. Blossom blight and typical anthracnose symptoms on fruit were observed in olive trees in an orchard in Rio Grande do Sul, Brazil. Fungi were isolated from symptomatic flowers. Fungal isolates were confirmed as pathogenic after Koch’s postulates were fulfilled. Blossom blight and typical anthracnose symptoms with salmon conidial masses were observed in flowers of cultivars Arbosana and Arbequina, at 96 h after inoculation (hai), and on fruits of cultivar Arbosana, at 120 hai. The identification of the pathogens was carried out using a polyphasic analysis approach, combining morphological and molecular characters of specific genomic regions (ITS, GAPDH, ACT, HIS3, and TUB2). Morphological characters and phylogenetic analyses revealed the isolates as Colletotrichum acutatum and Colletotrichum nymphaeae. Until now, the species C. nymphaeae had not been identified as causing anthracnose in olive trees in Brazil.
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Acknowledgements
Paula Freitas Filoda would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) for providing scholarships (Finance code 001). Leandro José Dallagnol was supported by fellowship CNPq (grant number 308149/2018-1).
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Filoda, P.F., Arellano, A.D.V., Dallagnol, L.J. et al. Colletotrichum acutatum and Colletotrichum nymphaeae causing blossom blight and fruit anthracnose on olives in southern Brazil. Eur J Plant Pathol 161, 993–998 (2021). https://doi.org/10.1007/s10658-021-02372-y
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DOI: https://doi.org/10.1007/s10658-021-02372-y