Abstract
A rapid method for colorimetric monitoring of bacterial viability is described. The colorimetric method was carried out based on glucose oxidase-encapsulated Zn/Co-infinite coordination polymer (Zn/Co-ICP@GOx), which was prepared in aqueous solution free of toxic organic solvents at room temperature. The Zn/Co-ICP@GOx was confirmed to be a robust sphere structure with an average diameter of 147.53 ± 20.40 nm. It integrated the catalytic activity of natural enzyme (GOx) and mimetic peroxidase (Co (П)) all in one, efficiently acting as a biocatalytic cascade platform for glucose catalytic reaction. Exhibiting good multi-enzyme catalytic activity, stability, and selectivity, Zn/Co-ICP@GOx can be used for colorimetric glucose detection. The linear range was 0.01–1.0 mmol/L, and the limit of detection (LOD) was 0.005 mmol/L. As the glucose metabolism is a common expression of bacteria, the remaining glucose can indirectly represent the bacterial viability. Hence, a Zn/Co-ICP@GOx-based colorimetric method was developed for monitoring of bacterial viability. The color was intuitively observed with the naked eye, and the bacterial viability was accurately quantified by measurement of the absorbance at 510 nm. The method was applied to determination of bacterial viability in water and milk samples with recoveries of 99.0–103% and RSD of 0.43–7.5%. The method was rapid (less than 40 min) and applicable to different bacterial species irrespective of Gram-positive and Gram-negative bacteria, providing a universal and promising strategy for real-time monitoring of bacterial viability.
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Funding
The authors gratefully acknowledge the financial support of this study by grants from the National Natural Science Foundation of China (21804025), the Guangdong Provincial Natural Science Foundation of China (2018A0303130079), the Science and Technology Program of Guangzhou, China (202103000089), the Project for Innovation and Strong School of Department of Education of Guangdong Province, China (2019GCZX012), the Industry University Research Collaborative Innovation Major Projects of Guangzhou Science Technology Innovation Commission, China (201604020164), the Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, China (2017GCZX002), and the Guangdong Demonstration Base for Joint Cultivation of Postgraduates.
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Qiu, P., Yuan, P., Deng, Z. et al. One-pot facile synthesis of enzyme-encapsulated Zn/Co-infinite coordination polymer nanospheres as a biocatalytic cascade platform for colorimetric monitoring of bacteria viability. Microchim Acta 188, 322 (2021). https://doi.org/10.1007/s00604-021-04981-0
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DOI: https://doi.org/10.1007/s00604-021-04981-0