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LncRNA ORLNC1 Promotes Bone Marrow Mesenchyml Stem Cell Pyroptosis Induced by Advanced Glycation End Production by Targeting miR-200b-3p/Foxo3 Pathway

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Abstract

Bone marrow mesenchymal stem cells (BMSCs) are a type of adult stem cells that originate from the mesoderm and have important roles in the body because of their self-renewal and multidirectional differentiation potential. Now it has been proved that BMSCs are closely related to the development of osteoporosis (OP). There is growing evidence that lncRNAs are involved in regulating the pyroptosis of BMSCs. And advanced glycation end-products (AGEs) have been recognized as NOD-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome activators. In this study, we aimed to explore the role of lncRNA ORLNC1 (NONMMUT016106.2) on the pyroptosis of BMSCs under CML (Nε-(carboxymethyl) lysine, the most common AGEs) treatment and its specific molecular mechanisms. Our study revealed that CML treatment promoted pyroptosis of BMSCs and upregulated ORLNC1 expression. As a competing endogenous RNA (ceRNA) of miR-200b-3p, the level of ORLNC1 was negatively correlated with miR-200b-3p. Foxo3 was a target of miR-200b-3p and ORLNC1 promoted BMSCs pyroptosis induced by CML through targeting miR-200b-3p/Foxo3 pathway.

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Data Availability

The data that support the findings of this study are available from the corresponding authors on reasonable request.

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Acknowledgements

This work was supported by 2019 Hebei Provincial Government-Funded Clinical Talents Training and Basic Research projects from China.(2019-14)We would like to thank all the researchers and study participants for their contributions.

Funding

This work was supported by 2019 Hebei Provincial Government-Funded Clinical Talents Training and Basic Research projects from China. (2019–14).

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Authors and Affiliations

  1. Department of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, Hebei Province, People’s Republic of China

    Lili Zhang & Yukun Li

  2. Department of Endocrinology, The Second Hospital of Shijiazhuang, Shijiazhuang, 050051, Hebei Province, People’s Republic of China

    Lili Zhang & Juan Li

  3. Department of Orthopedics, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, Hebei Province, People’s Republic of China

    Shilun Li

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  1. Lili Zhang
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  3. Juan Li
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  4. Yukun Li
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Contributions

Conceptualization: Lili Zhang; Data curation: Shilun Li; Formal analysis: Juan Li; Funding acquisition: Yukun Li; Methodology: Shilun Li; Project administration: Lili Zhang and Shilun Li; Writing—original draft: Lili Zhang; Writing—review & editing: Lili Zhang and Juan Li. All authors and participants reviewed the paper and approved the final manuscript.

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Correspondence to Yukun Li.

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All experimental processes were performed in strict compliance with the regulations of the Ethics Committee of Hebei Medical University. Also, all animal experiments were approved by the Ethics Committee of Hebei Medical University.

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Zhang, L., Li, S., Li, J. et al. LncRNA ORLNC1 Promotes Bone Marrow Mesenchyml Stem Cell Pyroptosis Induced by Advanced Glycation End Production by Targeting miR-200b-3p/Foxo3 Pathway. Stem Cell Rev and Rep 17, 2262–2275 (2021). https://doi.org/10.1007/s12015-021-10247-2

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