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Impact of microorganism inoculation on growth and Si accumulation in rubber seedlings

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Abstract

Microbial agents present in soil rhizosphere play a key role in reducing intensive use of inorganic fertilizers, which cause environmental pollution and negatively affect soil fertility. To improve plant growth, microbial agents with silicon (Si) can be used in combination with chemical fertilizers. In this glasshouse study, co-inoculants Enterobacter sp. UPMSSB7, mycorrhizae (Glomus mosseae) and addition of Si were evaluated for growth improvement of rubber seedlings. Results of this study indicated that co-inoculation significantly (P < 0.01) improved stem height, chlorophyll content, leaf area, root and shoot dry weight, total root length, root surface area, and root volume as compared to control treatment. The co-inoculation significantly (P < 0.01) increased Si content in shoot and root as well as leaf nutrients content (N, P, and K) as compared to control treatment. Si alone had no effect on seedling growth and nutrients content. The population of Enterobacter sp. UPMSSB7 in rhizosphere of seedlings as a result of co-inoculation, was not significantly different from Enterobacter sp. UPMSSB7 with Si treatment. The mycorrhizae root colonization and spore density were significantly (P < 0.01) increased in co-inoculation, compared to control and single inoculations with Si. Thus, our results suggested that co-inoculants Enterobacter sp. and mycorrhizae could be an eco-friendly approach for growth promotion of rubber seedlings.

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Acknowledgements

This research was funded by Institute of Plantation Studies, Universiti Putra Malaysia for the Geran Putra IPB (Sub-Project) research grant (Grant No. GP-IPB/2017/9523502). Authors also like to thank Bahauddin Zakariya University, Multan, Pakistan for funding first author via overseas Ph.D. scholarship program.

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Authors and Affiliations

  1. Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Imran Shabbir, Mohd Yusoff Abd Samad, Radziah Othman & Noraini Md Jaafar

  2. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mui-Yun Wong

  3. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Zulkefly Sulaiman

  4. Institute of Plantation Studies, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mohd Yusoff Abd Samad, Mui-Yun Wong & Zulkefly Sulaiman

  5. Department of Agronomy, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

    Imran Shabbir & Syed Asad Hussain Bukhari

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  1. Imran Shabbir
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Shabbir, I., Samad, M.Y.A., Othman, R. et al. Impact of microorganism inoculation on growth and Si accumulation in rubber seedlings . J Rubber Res 24, 501–509 (2021). https://doi.org/10.1007/s42464-021-00117-4

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