Abstract
A novel gram-negative, aerobic, pink, motile, gliding, rod-shaped bacterium, designated P51T, was isolated from saline silt samples in Yantai, China. It was able to grow at 4–42 °C (optimum 33 °C), pH 4.0–9.0 (optimum 7.0), and in 0–11.0% NaCl (optimum 4.0%, w/v). It grew at 4 °C, which was lower than the minimum temperature for related strains. The genome consisted of 4111 genes with a total length of 5 139 782 bp. The 16S rRNA gene sequence analysis indicated that strain P51T was a member of the genus Echinicola and most closely related to ‘Echinicola shivajiensis’. A genome analysis identified genes encoding proteins associated with carbon source utilisation, and the carotenoid biosynthesis and β-lactam resistance pathways. Strain P51T shared an average nucleotide identity value below 84.7%, an average amino acid identity value between 70.8 and 89.3%, and a digital DNA-DNA hybridisation identity of between 17.9–28.2% with closely related type strains within the genus Echinicola. The sole menaquinone was MK-7, and the major fatty acids were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), summed feature 4 (anteiso-C17:1 B and/or iso-C17:1 I), and summed feature 9 (iso-C17:1ω9c and/or 10-methyl C16:0). The polar lipids included one phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, three unidentified aminolipids, and one unknown lipid. The phenotypic, chemotaxonomic, and phylogenetic analyses suggest that strain P51T is a novel species of the genus Echinicola, for which the name Echinicola salinicaeni sp. nov. is proposed. The type strain was P51T (KCTC 82513T = MCCC 1K04413T).
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Data availability
All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain P51T is MW110902 and the number for the whole genome sequence is JACODZ000000000. Supplementary figures and Supplementary tables are available with the online version of this paper.
Abbreviations
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- MEGA:
-
Molecular evolutionary genetics analysis
- HPLC:
-
High performance liquid chromatography
- HEPES:
-
N-(2Hydroxyethyl)Piperazine-N′-2-ethanesulfonic acid
- CAPSO:
-
3-Cyclohexylamino-2-hydroxypropanesulfonic acid sodium salt
- MES:
-
2-(N-Morpholino) ethane-sulfonic acid
- PIPES:
-
Piperazine-1,4-Bisethanesulfonic acid
- Tricine:
-
N-[Tris(Hhydroxymethyl)Methyl] glycine
- KCTC:
-
Korean Collection for Type Cultures
- JCM:
-
Japan Collection of Microorganisms
- MCCC:
-
Marine Culture Collection of China
- GHs:
-
Glycoside hydrolases
- GTs:
-
Glycosyl transferases
- CBM:
-
Carbohydrate-binding modules
- ANI:
-
Average nucleotide identity
- AAI:
-
Average Amino Acid identity
- dDDH:
-
Digital DNA-DNA hybridization
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Acknowledgements
We thank Prof. Aharon Oren for his valuable help with naming the species.
Funding
This work was supported by the National Natural Science Foundation of China (31700116), the Natural Science Foundation of Shandong Province (ZR2017MC019), the China Postdoctoral Science Foundation (2183), and the Key Science and Technology Program of Weihai (1070413421511).
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HZ drafted the manuscript. CYS, HZ, YJY, and YXZ performed isolation, deposition and identification. RY, HZ and STY performed genome analysis. YXL revised the manuscript. YXZ designed all the experiments and supervised the manuscript.
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Zhao, H., Song, CY., Yin, R. et al. Echinicola salinicaeni sp. nov., a novel bacterium isolated from saltern mud. Antonie van Leeuwenhoek 114, 1915–1924 (2021). https://doi.org/10.1007/s10482-021-01650-3
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DOI: https://doi.org/10.1007/s10482-021-01650-3