Correction to: Nature Methods https://doi.org/10.1038/s41592-021-01187-3, published online 5 July 2021.

In this article, the last sentence of the second paragraph read, “To our knowledge, no framework exists to solve common problems such as the freeform appending of additional necessary sequences to probe sequences such as primer pairs for PCR amplification to support a broad range of experimental designs or the batched construction of probe sets against multiple targets in parallel.” To clarify the relationship between PaintSHOP and existing tools, this has been changed as follows: “We set out to create a free-to-use framework with genome-level scalability and a web-based design interface that could accommodate a broad variety of oligo FISH technologies.” The error has been corrected in the PDF and HTML versions of the article.