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miR-106b-5p upregulation is associated with microglial activation and inflammation in the mouse hippocampus following status epilepticus

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Abstract

To investigate the association of miR-106b-5p with neuroinflammation and microglial activation in a status epilepticus (SE) mouse model. We examined changes in the expression of microRNA-106b-5p (miRNA-106b-5p), repulsive guidance molecule A (RGMa), triggering receptor expressed on myeloid cells 2 (TREM2), and the microglia-related markers interleukin (IL)-1β, IL-4, IL-6, IL-10, inducible nitric oxide synthase (iNOS), and arginase-1 (Arg-1) in the mouse hippocampus of the lithium–pilocarpine-induced SE mouse model. Eighty-four female C57BL/6 mice were randomly divided into a normal control group (n = 12), and six SE groups (n = 12/group), which were monitored at 6 h and at 1, 3, 7, 14, and 21 days (d) post-SE induction. Unlike in the dentate gyrus, immunohistochemical staining revealed prominent neuronal swelling at 6 h, significant neuronal loss and apoptosis on day 3, and recovery by day 14 in the hippocampal cornu ammonis (CA)1 and CA3 pyramidal cells in SE mice. We noted elevated levels of miRNA-106b-5p and all microglia-related markers, which peaked at 3 days post-SE, except IL-4, which peaked at 7 days post-SE, indicating inflammation and microglial activation. RGMa and TREM2 levels decreased at 6 h post-SE. All markers but miRNA-106b-5p, RGMa, and TREM2 returned to baseline levels at 21 days post-SE. Dual luciferase reporter gene assay showed that microRNA-106b-5p can interact with RGMa. We observed that miR-106b-5p level increased while both RGMa and TREM2 levels decreased post-SE and showed associations with microglial activation and inflammation in the mouse hippocampus, suggesting their potential as SE therapeutic targets.

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The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

This work was supported by the National Key Research and Development Plan (Grant No. 2016YFC1306203).

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HW and TY conceived and designed research; TY, HF and DRD collected data and conducted research; TY and JJS analyzed and interpreted data; TY wrote the initial paper; HW revised the paper; HW had primary responsibility for the final content. All authors read and approved the final manuscript.

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Correspondence to Hua Wang.

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The authors declare that they have no conflicts of interest.

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All animal experiments were performed according to the National Institutes of Health guidelines for the Care and Use of Laboratory Animals (NIH publication 80-23, revised in 1996) and the necessary approval (2020PS505K) was obtained from the Animal Ethics Committee of Shengjing Hospital Affiliated with China Medical University.

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Communicated by Thomas Deller, M.D.

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Yu, T., Fu, H., Sun, JJ. et al. miR-106b-5p upregulation is associated with microglial activation and inflammation in the mouse hippocampus following status epilepticus. Exp Brain Res 239, 3315–3325 (2021). https://doi.org/10.1007/s00221-021-06208-3

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  • DOI: https://doi.org/10.1007/s00221-021-06208-3

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