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Identification and characterization of olfactory genes in the parasitoid wasp Diadegma semiclausum (Hellén) (Hymenoptera: Ichneumonidae)

Published online by Cambridge University Press:  03 September 2021

Basman H. Al-Jalely ,
Penghao Wang ,
Yalin Liao  and
Wei Xu Open the ORCID record for Wei Xu [Opens in a new window]
Basman H. Al-Jalely
Affiliation:
Food Futures Institute, Murdoch University, Perth, WA6150, Australia College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq
Penghao Wang
Affiliation:
Food Futures Institute, Murdoch University, Perth, WA6150, Australia
Yalin Liao
Affiliation:
Food Futures Institute, Murdoch University, Perth, WA6150, Australia
Wei Xu*
Affiliation:
Food Futures Institute, Murdoch University, Perth, WA6150, Australia
*
Author for correspondence: Wei Xu, Email: W.Xu@Murdoch.edu.au
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Abstract

Diadegma semiclausum is an important parasitoid wasp and widely used in the biological control of the diamondback moth, Plutella xylostella, one of the most destructive pests of cruciferous plants. Insect olfactory system is critical in guiding behaviors including feeding, mating, and oviposition, in which odorant binding proteins (OBPs) and odorant receptors (ORs) are two key components. However, limited attention has been paid to D. semiclausum olfactory genes. In this study, a transcriptome sequencing was performed on the RNA samples extracted from D. semiclausum male and female adult antennae. A total of 17 putative OBP and 67 OR genes were annotated and further compared to OBPs and ORs from P. xylostella, and other hemipteran parasitoid species. The expression patterns of D. semiclausum OBPs between male and female antennae were examined using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Six OBPs (DsemOBP 6, 7, 8, 9, 10, and 14) demonstrated significantly higher expression levels in females than in males, which may assist in female D. semiclausum host-seeking and oviposition behaviors. This study advances our understanding of the olfactory system of D. semiclausum at the molecular level and paves the way for future functional studies aiming at increasing the efficacy to control P. xylostella by using D. semiclausum.

Keywords

Diamondback mothgene expressioninsect olfactionodorant binding proteinodorant receptor
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 112 , Issue 2 , April 2022 , pp. 187 - 196
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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